摘要
目的 研究急性肝衰竭大鼠肝脏解偶联蛋白（UCP）2的表达及其与内毒索、氧自由基损伤的关系,进一步探讨肝衰竭的发病机制.方法将SD大鼠35只随机分为对照组（15只）、肝衰竭组（20只）.腹腔注射硫代乙酰胺（TAA）制备肝衰竭大鼠模型.测定各组大鼠血清内毒素（ET）水平,肝脏组织UCP2 mRNA的表达、丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性,免疫组织化学观察UCP2表达.组间比较采用Mann-Whitney U检验或单因素方差分析.结果肝脏内UCP2 mRNA、MDA高于对照组[分别为相对表达量0.41±0.04对比0.31±0.04和（6.69±1.15）nmol/mg对比（1.73±0.18）nmol/mg,P＜0.01]; ET水平高于对照组[（15.63±4.90）×10-3EU/ml对比（8.07±2.26）×10-3EU/ml,P＜0.01]; SOD含量低于对照组[（98.56±17.75）U/mg对比（246.87±51.27）U/mg,P＜0.01]; UCP2 mRNA的表达与ET、SOD、MDA有一定的相关性（r值分别为0.952,-0.667,0.634,P＜0.01或P＜0.05）;肝衰竭组UCP2表达增加.结论 肝衰竭组大鼠肝脏内UCP2表达增加,UCP2 mRNA与ET、SOD、MDA水平有一定的相关性.
Objective To investigate the relationship between uncoupling protein 2 （UCP2） expression and the damage caused by oxygen free radicals in acute liver failure rat models. Methods Thirty-five male Sprague-Dawley rats were randomly divided into two groups： the control group （15 rats） and liver failure group （20 rats）. The rats were injected intraperitoneally with thioacetamide （TAA） to induce models of acute liver failure. The levels of endotoxin （ET） were detected by double antibody sandwich enzyme-linked imrnunosorbent assay. The expression of liver UCP2 mRNA was detected by reverse transcription polymerase chain reactin. The superoxide dismutase （SOD） and malonaldehyde （MDA） were detected by spectrophotometry. The expression of UCP2 protein was observed by immunohistochemistry. The data of the two groups were compared using Mann-Whitney U test or ANOVA. Results The expression of UCP2 mRNA in liver failure group was higher as compared to the control group （P 〈 0.01）; the level of MDA and endotoxin of liver failure group were higher than that of the control group （P 〈 0.01）. SOD of the liver failure group was lower （P 〈 0.01）. There was a certain correlation between UCP2 mRNA expression and ET, SOD and MDA （r = 0.952, -0.667, 0. 634 respectively, P 〈 0.05 or 0.01）. Conclusions UCP2 is highly expressed in the livers of liver failure rats. A certain correlation perhaps existed between the expression of UCP2 mRNA and the seral SOD, MDA and ET.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2011年第1期55-57,共3页
Chinese Journal of Hepatology
关键词
肝衰竭
急性
内毒素血症
氧自由基
解偶联蛋白
Liver failure, acute
Endotoxemia
Oxygen free radicals
Uncoupling protein
