摘要
目的观察黄芪对高游离脂肪酸(FFA)致大鼠主动脉内皮依赖性血管舒张功能(EDV)损伤的保护作用。方法取SD雄性大鼠(n=11)胸动脉制成主动脉环,将血管环置于3个器官池浴槽中,分别加入20mL Krebs-Henseleit(K-H)液(空白对照组)、20mL K-H液与0.833mL棕榈酸(800μmol/L)的混合液(FFA组)、20mL K-H液与0.833mL棕榈酸和42μL的黄芪液(4g/L)混合液(FFA+黄芪组),观察主动脉环对乙酰胆碱(Ach)和硝普钠(SNP)的舒张反应,高效液相色谱法测定各组器官池液中一氧化氮的代谢产物(NOx)水平,免疫组化检测主动脉环内皮细胞核因子-κB(NF-κB)的表达。结果 FFA组与对照组相比,EDV受损(P<0.05)。而FFA+黄芪组与FFA组相比,EDV改善(P<0.05)。3组血管环在舒张反应达峰值时器官浴槽K-H液中NOx的水平分别是(104.1±14.2)μmol/g(对照组)、(83.1±8.4)μmol/g(FFA组)、(98.8±10.7)μmol/g(FFA+黄芪组),FFA组低于对照组(P<0.05),FFA+黄芪组高于FFA组(P<0.05)。免疫组化结果显示,大鼠FFA组NF-κB表达较对照组多,FFA+AM组较FFA组少。结论 FFA水平对主动脉内皮依赖性血管舒张功能具有损伤作用,而黄芪能缓解FFA水平对血管内皮功能的损伤,对血管内皮细胞具有保护作用。
Objective To investigate whether Astragalus membranaceus（AM） can protect endothelium-dependent vasodilatation（EDV） function of aorta from the damage induced by high level of free fatty acid（FFA）.Methods Ten male SD rats,8 weeks old and 250-300 g in weight,were sacrificed and thoracic aorta were harvested.Aorta rings incubated in organ baths were divided into three groups,Control group,FFA group and FFA＋AM group.The control group was incubated in 20mL Krebs-Henseleit solution;the FFA group was incubated in 20 mL KH solution mixed with FFA（800 μmol/L）;the FFA＋AM group was incubated in 20mL KH solution mixed with FFA（800 μmol/L）and AM（4 g/L）.The relaxation levels of aorta rings response to acetylcholine and sodium nitroprusside were measured,the expression of NF-κB and the level of NOx in the organ bath were analyzed by immunohistochemistry.Results Severe endothelial dysfunction were induced in FFA group（maximal vasorelaxation in response to Ach：61.1%±6.9% vs.93.1%±2.7% in control,P〈0.05）,while EDV in FFA＋AM group was significantly improved by the incubation with AM（P〈0.05）.Compared with the control group（104.1±14.2） μmol/g,NOx levels of FFA group was（83.1±8.4） μmol/g（P〈0.05）,and the treatment of AM increased the levels of NOx（98.8±10.7）μmol/g（P〈0.05）.The control vascular ring had a little NF-κB expression in endothelial nucleus,FFA increased the activation of NF-κB,while the treatment of AM lower the elevated NF-kB level.Conclusion FFA can directly injure EDV,while AM may ameliorate it,with the possible mechanism related to the signal pathway of NF-κB and NO.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2011年第1期48-51,共4页
Journal of Sichuan University(Medical Sciences)
关键词
高游离脂肪酸
黄芪
主动脉环
High free fatty acid Astragalus membranaceus Aorta rings
