摘要
研究了长江、辽河、瓯江水系野生中华绒螯蟹(Eriocheir sinensis)及其莱茵河水系F1共4个群体71个个体的线粒体细胞色素氧化酶亚基I(COI)基因序列,比对长度包括628个位点,其中变异位点112个,简约信息位点36个。基因序列G+C(%)含量较低,表现出较为明显的碱基组成偏倚性。71个个体共含17种单倍型,单倍型H13出现次数最多,为莱茵河水系F1、长江以及瓯江群体共享;单倍型H15、H16和H17为瓯江群体特有。样本总体遗传多样性指数较高,其单倍型多样性指数(H)为0.862,核苷酸多样性指数(π)为0.016 9。各水系野生群体遗传多样性分析表明,长江群体单倍型多样性指数最高(0.838 0),莱茵河水系F1群体最低(0.725 0);长江群体与辽河群体间遗传距离最小(0.002 44),推测长江与辽河2群体间基因交流较多;长江群体内遗传距离(0.017 38)大于除瓯江外的其他水系群体内遗传距离,揭示长江群体内遗传变异较大、种质混杂较严重。本研究中各群体间的基因流(Nm)均高于1,其中长江群体与辽河及莱茵河F1群体间的基因流分别为8.27和9.72,表明长江群体与这2个群体间曾经有较为频繁的基因交流。分子变异分析(AMOVA)结果显示,4个群体总的遗传分化指数为0.268 5(P<0.001),其中90.77%遗传变异存在于各群体内部,9.23%变异存在于群体间。最小跨度网络图和系统发育分析均没有显示出单倍型与地理位置的对应关系,已不能形成明显的地理群体结构,证明当前各水系中华绒螯蟹种质混杂确实存在。
Sequences of mitochondrial COI gene of 71 individuals of Eriocheir sinensis which were sampled from Yangtze River,Liao River,Ou River population and F1 generation of Rhine River population were determined.The result showed that there were 112 variable sites population and 36 parsimony-informative sites in total 628 sites.The content of（G＋C） was lower,showing obvious bias of base composition.Seventeen haplotypes were detected among the 71 samples.Haplotype H13 which occurred most frequently was shared by the populations from Yangtze River,Ou River,and F1 generation of Rhine River population.Haplotypes H15,H16 and H17 were unique in the population from Ou River.Haplotype diversity index（H） was 0.862 and nucleotide diversity index（π） was 0.016 9 indicating that the genetic diversity index of total samples was high.Yangtze River population had the highest（0.838） while the F1 generation of Rhine River population had the lowest（0.725 0） haplotype diversity index among the four populations.The genetic distance between Yangtze River population and Liao River popula-tion was closer than that among others and it can be inferred that the genetic exchange between them was more frequently.The genetic distance within Yangtze River population（0.017？38） was higher than those of others ex-cept of Ou River population which indicating that higher genetic variation and more serious genetic admixture exist within Ou River population.Gene flows（Nm） among four populations were all above 1,especially between Yangtze River population and Liao River population（8.27） and between Yangtze River and F1 generation of Rhine River（9.72）,respectively,which indicated that the population of Yangtze River had frequent gene flow with the other two before.Analysis of molecular variance（AMOVA） showed that genetic differentiation index of all the four populations was 0.2685（P〈0.001）,of which the majority（90.77%） existed within populations,and the rest existed among populations（9.23%）.Haplotype n
出处
《中国水产科学》
CAS
CSCD
北大核心
2011年第1期16-22,共7页
Journal of Fishery Sciences of China
基金
国家"十一五"科技支撑计划项目(2006BAD01A1305)
江苏省高新技术研究项目(BG2004319)
江苏省水产三项工程项目(K2008)
江苏省科技支撑计划(农业)项目(BE2009336)
