摘要
[目的]吸水链霉菌A03是自主分离的高效拮抗菌株,其活性代谢产物对多种植物病原真菌具有强烈抑制作用;为了建立其高产发酵工艺,并为过程放大提供技术参数,利用正交设计和单因素试验对其发酵培养基和培养条件进行优化。[结果]获得的最适发酵培养基配方(g/L):葡萄糖5,可溶性淀粉5,黄豆粉10,磷酸二氢钾0.5,碳酸钙1,七水硫酸镁0.1;最优培养条件:液体种子菌龄40 h,500 mL摇瓶装液量80 mL,接种量为体积分数12%,摇床转速180 r/min,培养温度28℃,发酵周期216 h;发酵液抑菌直径达3.04 cm。[结论]成功建立了该菌株的小试发酵工艺,发酵水平提高了52.0%,发酵周期缩短30.77%。研究结果为其发酵代谢调控和过程放大提供了基本参数。
[Aims]Streptomyces hygroscopicus A03 is an antagonistic strain isolated by this research group.In previous studies,the active metabolite of the strain presented a significant inhibitory effect against many phytopathogenic fungi.In order to develop an efficient fermentation process for producing antifungal substances with Streptomyces hygroscopicus A03 and provide technical parameters for the further scale up of the process,the medium components and culture conditions were optimized with methods of orthogonal design and mono-factor test in this paper.[Results] The following optimal formula of liquid production medium was obtained： glucose 5 g/L,soluble starch 5 g/L,soybean meal 10 g/L,KH2PO4 0.5 g/L,CaCO3 1 g/L and MgSO4·7H2O 0.1 g/L.The suitable culture conditions were as follows： the liquid seed was cultured for 40 hours,the fermentation was carried out in 500 mL erlenmeyer flask with 80 mL production medium inoculated with 12%（volume fraction） seed culture and incubated at 28 ℃,180 r/min for 216 hours.The inhibition zone produced by the filtrate of the fermented broth was 3.04 cm in diameter.[Conclusions] A lab-scale fermentation process for strain A03 was successfully established in the study.The fermentation level with the optimized process was 52.0% higher,and the fermentation period was 33.77% shorter than that by the basic fermentation process.The study result provided a set of valuable parameters for the metabolic control and scale up of the fermentation process of strain A03.
出处
《农药》
CAS
北大核心
2011年第2期93-96,共4页
Agrochemicals
基金
北京市自然科学基金资助项目(6101001)
公益性行业(农业)科研专项(200903049-07)
北京市农林科学院项目(2010A006)
关键词
吸水链霉菌
抗菌活性产物
发酵培养基
培养条件
Streptomyces hygroscopicus
antifungal metabolite
fermentation medium
culture condition
