摘要
通过正茬剪叶模拟连作地黄光合源不足条件下,测定分析植株源与库的关系,应用同位素示踪技术分析连作地黄光合产物的分配及内源激素的变化,以探讨地黄的连作障碍机制。结果表明:剪叶处理对植株的影响不能达到连作障碍程度,其库容及其活性大于连作地黄;连作地黄出现光合产物滞留于地上部,库的活性影响了植株光合产物向库的运输,在生长前期植株内源激素发生了变化;因此断定,连作地黄库容及其活性过低是限制地黄块根膨大的重要因素,库通过不同激素对源的反馈调节,抑制了地上源的光合能力及光合产物向库的分配,导致连作障碍。
In this study,the currently cropped Rehmannia glutinosa was leaf-clipped to simulate the shortage of photosynthate in successively cropped R. glutinosa,and 14C-istope tracer and ELISA techniques were adopted to analyze the distribution of photosynthate and the levels of endogenous hormones in successive R. glutinosa,aimed to approach the source-sink relationship of the phosynthate and to explore the mechanism of continuous cropping obstacle of R. glutinosa. Leaf-clipping did not have the same impact as continuous cropping obstacle. The volume and activity of phosynthate pool under leaf-clipping were bigger than those under continuous cropping. The photosynthate in continuously cropped R. glutinosa was remained in shoot,suggesting that the low activity of the sink affected the transportation of the photosynthate. The levels of endogenous hormones also changed at the early growth stage of continuously cropped R. glutinosa. Our results suggested that the small volume and low activity of the sink could be an important factor limiting the development of R. glutinosa roots in continuous cropping. The feedback regulation of the sink to source by endogenous hormones inhibited the photosynthesis capacity and the partitioning of photosynthate to sink,resulting in the continuous cropping obstacle.
出处
《生态学杂志》
CAS
CSCD
北大核心
2011年第2期248-254,共7页
Chinese Journal of Ecology
基金
国家自然科学基金项目(30772729
30973875和81072983)
国家"十一五"科技支撑计划项目(2006BA109B03)资助
关键词
地黄
连作障碍
14C光合产物分配
内源激素
源库关系
Rehmannia glutinosa
continuous cropping obstacle
14C photosynthate distribution
endogenous hormone
source-sink relationship.
