摘要
目的探讨维生素A对小鼠外周血及肠黏膜T淋巴细胞亚群发育及肠黏膜分泌细胞因子水平的影响。方法幼鼠共20只,随机分为高维生素A组(维生素A250IU/g)及对照组(维生素A4IU/g),每组10只,干预3周后应用流式细胞术测定小鼠外周血及肠黏膜CD4+CD25+T细胞亚群,应用ELISA法测定小鼠粪便中细胞因子IFN-γ、IL-4、IL-17及IL-23水平。结果高维生素A组外周血及肠黏膜CD4+CD25+T细胞水平高于对照组(P<0.05),粪便中IL-4水平高于对照组(P<0.05),IL-23水平低于对照组(P<0.05)。结论维生素A对小鼠外周血及小肠CD4+CD25+T细胞发育有促进作用,可能通过调节IL-4及IL-23等细胞因子参与肠黏膜相关免疫反应。
Objective To study the effects of vitamin A on the development of T lymphocytes in peripheral blood and small intestine and on the cytokine response of intestinal mucosa in mice.Methods Twenty young mice were randomly fed with forage containing vitamin A 250 or 4 IU/g（n=10 each）.Three weeks later,the levels of CD4＋ CD25＋ T subsets in peripheral blood and intestinal mucosa were measured by flow cytometry.The levels of cytokines IFN-γ,IL-4,IL-17 and IL-23 in stool were measured using ELISA.Results The levels of CD4＋ CD25＋ T subsets in peripheral blood and intestinal mucosa in the 250 IU/g vitamin A group were significantly higher than those in the 4 IU/g vitamin A group（P0.05）.The IL-4 level in stool increased,in contrast,the IL-23 level in stool decreased significantly in the 250 IU/g vitamin A group when compared with the 4 IU/g vitamin A group（P0.05）.Conclusions vitamin A may promote the development of CD4＋ CD25＋ T lymphocytes in peripheral blood and small intestine.Moreover,it may be involved in intestinal mucosa-associated immune response by regulating cytokines IL-4 and IL-23.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2010年第12期976-978,共3页
Chinese Journal of Contemporary Pediatrics
基金
上海市卫生局青年科研基金资助项目(2007Y80)
