摘要
目的:建立气相色谱测定火麻仁中亚油酸、α-亚麻酸的含量方法,为评价质量和标准制定提供依据。方法:ZB-WΑX毛细管柱(0.5μm×0.32 mm×30 m),载气为高纯度氮气,流速1.0 mL.min-1,分流比5∶1,柱温为190℃,进样口温度为250℃,FID检测器温度为250℃,空气450 mL.min-1,氢气45 mL.min-1。结果:火麻仁中亚油酸、α-亚麻酸含量分别31.42%～37.21%,8.02%～9.65%。结论:该法准确,简便,重复性好,可用于火麻仁中亚油酸、α-亚麻酸的质量控制。
Objective ：To establish the method for determination of the content of linoleic acid and α-linoleic acid in hemp fruit by GC, and provide evidence for evaluating quality and criterion. Method ： The GC separation was performed on a ZB-WAX column （0. 5 μm ×0. 32 mm ×30 m） at 190 ℃ , inject temperature at 250 ℃ , FID detecter temperature was at 250 ℃, gas as N2 （ 99.99% ） ; flow rate was 1.0 mL· min -1, air 450 mL. min · 1, H2 45 mL·min-1. Result： The content of linoleic acid and α-linoleic acid in hemp fruit was 31.42% -37.21% , 8.02% - 9.65%. Conclusion： The method can be used to control the quality of linoleic acid and α-linoleic acid in hemp fruit, which is accurate, convenient and repeatable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第3期83-85,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
甘肃省科学技术攻关计划项目(GS035-A43-048-03)
