摘要
目的探讨非小细胞肺癌(NSCLC)组织中趋化因子受体(CXCR4)的表达与肺癌生物学行为以及血管形成之间的关系,着重分析CXCR4的表达以及新生血管形成在肺癌侵袭、转移及预后中的生物学意义。方法应用免疫组织化学方法 (SP法)检测62例非小细胞肺癌组织、12例癌旁正常组织中CXCR4的表达及微血管密度(MVD)的值,并分析CXCR4的表达及MVD与组织类型、肿瘤大小、TNM分期、分化程度、淋巴结转移的相关性。结果 CXCR4在肺癌组织中的阳性表达率为58.1%,在正常组织中的阳性表达率为16.7%,两者比较差异有显著性(P<0.05)。非小细胞肺癌组织中CXCR4表达明显升高,以及MVD明显高于正常组织,与肿瘤的分化程度、临床分期、淋巴结转移均有相关性(P<0.05),与肿瘤的大小、病理分型无关(P>0.05)。CXCR4在NSCLC中的表达以及肿瘤MVD二者呈显著正相关。结论同时检测非小细胞肺癌组织的CXCR4的表达及MVD对判断肿瘤的恶性程度和估计预后有一定的意义。
Objective To investigate correlations between expression of CXCR4,MVD and clinicalbiological characteristics,to analyze the relationship with invasion,metastasis and prognosis in non-small cell lung cancer.Methods High sensitive S-P immunohistochemical method was used to detect the expressions of CXCR4 and MVD in 62 cases of NSCLC and 12 cases of para-cancerous normal lung tissues.To study the relationships between their expressions and histological types of NSCLC,Tumor size,TNM stages,differentiations,lymph node metastasis and their correlations each other.Results The positive rates of CXCR4 expression was 58.1% in NSCLC,which was dramatically higher than that in a normal tissues（16.7%）（P0.05）.The expressions of CXCR4 and MVD was significantly correlated in NSCLC to TNM stage,differentiations and lymph node metastasis（P0.05）.It was no relationship with the histological types and tumor size of NSCLC（P0.05）.CXCR4 play important roles in non-small cell lung cancer procession,especially in tumor metastasis.CXCR4 have intimate relationship with MVD.Conclusion CXCR4 and MVD may be good preditors of metastasis and prognostic evaluation for NSCLC patients.
出处
《中国实验诊断学》
北大核心
2011年第1期72-75,共4页
Chinese Journal of Laboratory Diagnosis
