摘要
目的建立HPLC法测定川贝定喘胶囊中橙皮苷含量的方法。方法采用Agilent Eclipse XDB-C18色谱柱,甲醇-2%醋酸(35:65)为流动相;流速1.0ml·min-1;检测波长283nm。结果橙皮苷在0.4064～3.251μg范围内线性关系良好(r=0.9998),平均回收率为98.82%,RSD=1.12%(n=5)。结论本方法操作简便,专属性强,结果准确,可用于川贝定喘胶囊的质量控制。
Objective：To establish a HPLC method for the determination of Orange popeel glucoside in chuanbei Dingchuan capsules.Method：The chromatographic system consisted of C18column,methanol-2% acetic acid（35：65） as mobile phase.The flow rate was 1.0ml ·min^-1,and the detecting wavelength was 283nm.Results：The Orange popeel glucoside have a good liner relation in the range of0.4064 ～ 3.251μg（r = 0.9998）,the average recovery was 98.82% and RSD = 1.12%（n = 5）.Conolusion：The established method is accuracy,sensitive and simple.It can be used for quality control of chuanbei Dingchuan capsules
出处
《光明中医》
2011年第1期58-59,共2页
GUANGMING JOURNAL OF CHINESE MEDICINE
