摘要
以铁皮石斛组培苗为材料,研究不同蔗糖浓度对铁皮石斛原球茎(PLB)生长的影响,6-BA用量对铁皮石斛增殖芽生长的影响,不同糠源及添加物香蕉对铁皮石斛壮苗、生根的影响。结果表明:当蔗糖30g/L最有利于铁皮石斛PLB增殖,最适合PLB的培养基为MS+NAA 0.1mg/L+蔗糖30g/L。6-BA对铁皮石斛丛生芽形态建成有着很重要的作用,有利于促进芽的分化,适合继代的培养基为MS+BA 4mg/L+NAA0.1mg/L+CW5%+蔗糖30g/L。香蕉对铁皮石斛组培苗的根系生长具有显著的促进作用,以50 g/L葡萄糖为糖源更有利于铁皮石斛壮苗生长,适合生根苗生长的培养基为1/2MS+香蕉70g/L+活性炭0.1%+葡萄糖50g/L。
Tissue culture seedings of Dendrobiurn candidum were used to study the effect of sucrose on PLBs growth, and the effect of 6-BA on shoot proliferation and growth, and the effect of carbon sources and banana on cultivating strong seedlings and rooting. The results showed that growth status of PLBs was best when the concentration of sucrose was 30 g/L. The most suitable medium for PLBs was MS＋NAA 0. 1 mg/L＋sucrose 30 g/L. 6-BA plays a very important role in buds morphogenesis. The suitable medium for the seedlings subculture was MS＋BA 4 mg/L＋NAA 0. 1 mg/L＋CW 5%＋ sucrose 30 g/L. Banana promoted the root of Dendrobium officinale Kirnura et Migo growth significantly. Medium plus 50 g/L glucose as carbon source was better for the growth of Dendrobium candidurn plantlets. The suitable medium for growth and rooting was 1/2MS＋banana 70 g/L＋activated carbon 0. 1＋glucose 50 g/L.
出处
《北方园艺》
CAS
北大核心
2011年第2期146-148,共3页
Northern Horticulture
基金
福建省省院科技合作专项(农业)资助项目(2009N4008)
关键词
铁皮石斛
碳源
6-BA
Dendrobium officinale Kimura et Migo
carbon source
6-BA
