摘要
本文比较了不同黑曲霉(Aspergillus niger)菌株对大豆田除草剂氯嘧磺隆的耐药性,并且对抗性菌株中靶标酶乙酰乳酸合成酶(ALS)的酶学特性进行了研究。结果发现TR-H为抗性菌株,其ALS酶活性显著高于其它菌株。酶学特性分析显示,TR-H菌株的最佳培养时间为84h,最佳反应温度为35℃,最适反应pH为7.3,酶反应的初速度范围为0～60min。以丙酮酸钠为反应底物时,其米氏常数(Km)为39.41mmol/L,最大反应速度(Vmax)为232.2μg/mL·h。
Tolerance of different Aspergillus niger strains to herbicide chlorimuronethyl was investigated in soybean field.The ALS target enzyme characteristics of the resistant strains were also studied.Results showed a resistant strain TRH and its ALS enzyme activity was significantly higher than other strains.Enzymatic characteristics constant showed that the optimum culture time was 84h at 35℃ with an initial velocity of 0 to 60min,pH7.3.When the substrate was sodium pyruvate,the Michaelis constant （Km） of ALS was 39.41mmol/L with the maximal reaction velocity （Vmax） of 232.2μg/mL·h.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2010年第4期563-566,共4页
Chinese Journal of Oil Crop Sciences
基金
农业部转基因生物新品种培育重大专项(2008ZX08004-001-20)
黑龙江省科技计划项目(GB03B711-02)
关键词
氯嘧磺隆
黑曲霉
乙酰乳酸合成酶
酶学特性
Chlorimuronethyl
Aspergillus niger
Acetolactate synthase
Enzyme characteristics
