摘要
以天然提取的山奈酚粗品为原料，对其进行纯化工艺的研究以获得山柰酚标准品。采用冰醋酸作为结晶溶剂，对山柰酚进行纯化精制，并采用HPLC方法对其进行含量测定，以Kromasil KR100-5C18（250mm×4．6mm，5μm）为色谱柱，乙腈-0．1％磷酸溶液（35：65）为流动相，流速1．0mL μmin^-1，柱温30℃，检测波长366nm。采用面积归一化法测得产品含量为99．70％，收率为67％。山柰酚的线性范围在5—500μg·mL^-1（r=0．9997）。该方法操作简单，实用性强，为大规模制备山奈酚标准品提供了参考方法。
To obtain standad sample of kempferol from natural extracted sample, acetic acid was used as reerystalization. A kromasil KR100-5 C18 column（was 250 mm×4.6 mm,5 μm） used with a mixture of acetonitrile and 0. 1% phosphoric acid solution （35：65） as the mobile phase at a flow rate of 1.0 mL/min,the column temperature was 30 ℃ with the detection wavelength at 366 nm. The purification yield was above above 67%, and the content was up to 99.70%. The linear curve was obtained in the range of 5 - 500μg·mL^-1（ r = 0. 9997 ）. This method is simple, practical, and it provides a way to prepare high purity kaempferol for industry.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第B08期106-108,共3页
Natural Product Research and Development
基金
科技部科技基础性工作专项重点项目（2007FY130100）
卫生公益性行业科研专项经费（200802038）
关键词
山柰酚
纯化
重结晶
HPLC
标准品
kaempferol
purity
recrystallization
HPLC
standad sample
