摘要
目的观察转染pCE质粒的干细胞对肝癌的治疗作用。方法采用尾静脉直接注射法治疗大鼠肝脏转移瘤,设立阴性对照、干细胞、转染pCE质粒的干细胞组和5-Fu组,通过观察转移瘤在肝脏组织中的生长情况和卫星灶形成,肝脏组织的病理学改变,检测基质衍生因子-1(stromal cell de-rived factor-1,SDF-1)表达,比较4组对肝癌的治疗作用。结果转染pCE质粒的干细胞组对肿瘤细胞的生长抑制率(56%)与阴性对照组(0)、干细胞组(8%)和5-Fu组(78%)比较差异有统计学意义(P<0.05)。治疗前4组SDF-1的表达差异无统计学意义(P>0.05)。转染pCE质粒的干细胞组SDF-1在治疗后分泌增加(OD=0.48),与阴性对照组(OD=0.35)、干细胞组(OD=0.39)和5-Fu组(OD=0.36)比较差异有统计学意义(P<0.05)。结论转染pCE质粒的干细胞有抑制肝癌生长的作用,SDF-1表达水平上升是其机制之一。
Objective To observe stem cells with pCE plasmid therapy for liver cancer,and explore its related mechanism.Methods After injection of stem cells into tail vein,inhibiting tumor ratio was calculated,satellite lesions in the liver,change of tumor mass in pathology,expression of SDF-1 were studied.The difference among control group,group of stem cells,group of stem cells with pCE plasmid,and group of 5-Fu were compared.Results Stem cells with pCE plasmid could inhibit proliferation of cancer cells （56%）in the following 10 day（P0.05）,compared with control group（0%）,group of stem cells（8%） and group of 5-Fu（78%）,respectively.Expression of SDF-1 from group of stem cells with pCE plasmid after treatment was increased （OD=0.48）,different from control group（OD=0.35）,group of stem cells（OD=0.39） and group of 5-Fu（OD=0.36） （P0.05）.Expression of SDF-1 from all groups before treatment did not have difference（P0.05）.Conclusion Stem cells with pCE plasmid could inhibit proliferation of cancer cells through the help of SDF-1.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第12期1370-1373,共4页
Cancer Research on Prevention and Treatment
基金
武汉市科技局晨光计划科研基金资助项目(200750731292)
