期刊文献+

北京地区福氏志贺菌4c亚型毒力基因分析 被引量:2

Detection of virulence genes of Shigella flexneri 4c isolated in Beijing
原文传递
导出
摘要 目的了解北京地区福氏志贺菌4 c亚型生化特征和毒力基因携带情况。方法对分离自北京地区的44株福氏4 c亚型志贺菌采用Api生化板条进行鉴定,玻片凝集法进行血清分型,应用PCR技术对其侵袭性质检抗原(ipaH)、志贺肠毒素(setlA、setlB)、侵袭相关位点(sen、ial)和毒力表达调控基因(virF)6种毒力基因进行检测。结果全部福氏4 c志贺菌只发酵葡萄糖、甘露醇、蜜二糖和阿拉伯糖;血清凝集抗原结构式为(Ⅳ:7,8);ipaH、setlA、setlB、sen、ial和virF6毒力基因携带率分别为100%、97.7%、100%、77.2%、72.7%和77.2%;54.5%的菌株携带全部6种毒力基因。结论福氏4 c志贺菌毒力基因携带率高,具有较强致病性。 Objective To investigate and analyze the biochemical characteristics and virulence genes of Shigella flexneri 4c isolated in Beijing.Methods Totally 44 S.flexneri 4c isolates from diarrhea patients in Beijing were systematically determined with biochemical reactions and serological tests.Detections of ipaH,setlA,setlB,sen,ial,and virF virulence genes were performed using PCR.Results All the S.flexneri 4c isolates could ferment glucose,mannitol,melibiose,and arabinose.Presence of ipaH,setlA,setlB,sen,ial,and virF virulence genes were identified among 100%,97.7%,100%,77.2%,72.7%,and 77.2% of 44 tested isolates,respectively.Conclusion Presence of virulence genes associated with strong pathogenicity was found in a high proportion of Shisella flexneri 4c isolates.
出处 《中国公共卫生》 CAS CSCD 北大核心 2011年第1期46-47,共2页 Chinese Journal of Public Health
基金 国家科技重大专项(2008ZX10004-0082009ZX10004-2052009ZX10004-315) 国家自然科学基金(81001267/H2609)
关键词 福氏4c志贺菌 生化特征 毒力基因 Shigella flexneri 4c biochemical characteristics virulence gene
  • 相关文献

参考文献10

二级参考文献43

共引文献87

同被引文献19

  • 1何水渊,张建群,罗学辉,张怡明.福氏4c型志贺菌引起痢疾暴发病原学检测分析[J].中国卫生检验杂志,2006,16(7):887-887. 被引量:8
  • 2许学斌,袁政安,顾宝柯,金汇明,林亚萍,陈敏,朱超.上海市沙门菌和志贺菌腹泻型谱和耐药特征的分析[J].中国人兽共患病学报,2007,23(7):706-709. 被引量:11
  • 3叶应妩,王毓三,申子瑜.全国临床检验操作规程[M].3版.南京:东南大学出版社,2003:75. 被引量:17
  • 4Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susee-ptibility testing. Sixteenth informational supplement[S]. CLSI, 2006, M100-S 16:31. 被引量:1
  • 5Yavzori M, Cohen D, Orr N. Prevalence of the genes for shigella enterotoxins 1 and 2 among clinical isolates of shigella in Israel[ J]. Epidemiol Infeet, 2002, 128(3) : 533 -535. 被引量:1
  • 6Venkatesan MM, Buysse IM, Kopecko DJ. Use of Shigella flexneri ipaC and ipaH gene sequences for the general identification of Shi- geUa spp. and enteroinvasive Escherichia eoli[ J ]. J Clin Microbial, 1989, 27(12): 2687-2691,. 被引量:1
  • 7Vargas M, Gascon J, Jimenez De Anta MT, et al. Prevalence of Shigella enterotoxins 1 and 2 among Shigella strains isolated from patients with traveler' s diarrhea [ J ]. J Clin Microbiol, 1999, 37 (11) : 3608 -3611. 被引量:1
  • 8Sharma A, Singh SK, Kori L. Molecular epidemiological character- istics of Shigella spp. isolated from river Narmada during 2005 - 2006[J~. J Environ Health, 2009, 71(6) : 61 -66. 被引量:1
  • 9中华人民共和国卫生部.WS287--2008细菌性和阿米巴性痢疾诊断标准[s].北京:人民卫生出版社,2009. 被引量:2
  • 10Clinical and Laboratory Standards Institute. M100 - $20 Perform- ance standards for antimicrobial susceptibility testing: twentieth in- formational supplement [ S ]. Wayne, PA : Clinical and Laboratory Standards Institute, 2010. 被引量:1

引证文献2

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部