摘要
目的了解北京地区福氏志贺菌4 c亚型生化特征和毒力基因携带情况。方法对分离自北京地区的44株福氏4 c亚型志贺菌采用Api生化板条进行鉴定,玻片凝集法进行血清分型,应用PCR技术对其侵袭性质检抗原(ipaH)、志贺肠毒素(setlA、setlB)、侵袭相关位点(sen、ial)和毒力表达调控基因(virF)6种毒力基因进行检测。结果全部福氏4 c志贺菌只发酵葡萄糖、甘露醇、蜜二糖和阿拉伯糖;血清凝集抗原结构式为(Ⅳ:7,8);ipaH、setlA、setlB、sen、ial和virF6毒力基因携带率分别为100%、97.7%、100%、77.2%、72.7%和77.2%;54.5%的菌株携带全部6种毒力基因。结论福氏4 c志贺菌毒力基因携带率高,具有较强致病性。
Objective To investigate and analyze the biochemical characteristics and virulence genes of Shigella flexneri 4c isolated in Beijing.Methods Totally 44 S.flexneri 4c isolates from diarrhea patients in Beijing were systematically determined with biochemical reactions and serological tests.Detections of ipaH,setlA,setlB,sen,ial,and virF virulence genes were performed using PCR.Results All the S.flexneri 4c isolates could ferment glucose,mannitol,melibiose,and arabinose.Presence of ipaH,setlA,setlB,sen,ial,and virF virulence genes were identified among 100%,97.7%,100%,77.2%,72.7%,and 77.2% of 44 tested isolates,respectively.Conclusion Presence of virulence genes associated with strong pathogenicity was found in a high proportion of Shisella flexneri 4c isolates.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第1期46-47,共2页
Chinese Journal of Public Health
基金
国家科技重大专项(2008ZX10004-0082009ZX10004-2052009ZX10004-315)
国家自然科学基金(81001267/H2609)
关键词
福氏4c志贺菌
生化特征
毒力基因
Shigella flexneri 4c
biochemical characteristics
virulence gene