摘要
采用抽吸法和切割法两种采卵方法收集马卵母细胞,显示采用切割法的卵母细胞回收率为83%,高于抽吸法的回收率46.5%(P<0.05),在卵母细胞的成熟上,使用M199和DMEM/F12两种培养液为基础液的成熟体系,紧凑型(Cp)COCs和扩展型(Ex)COCs在以M199为基础液和以DMEM/F12为基础液的培养液中的卵母细胞成熟率分别为41.7%和64.7%、46.7%和66.7%,差异不显著(P>0.05)。两种培养体系中成熟的CpCOCs采用Ion-omycin与6-DMAP和CHX联合激活,在以M199和以DMEM/F12为基础液的培养液中成熟的卵母细胞的卵裂率分别为45%和57.1%,差异不明显(P>0.05)。
In this study, two different methods （ aspiration and incision） were evaluated for efficiency of equine oocyte collection. Two maturation media were used for in vitro maturation of equine oocytes. In medium 1, the basic medium was M199; in medium 2, the basic medium was DMEM/F12. The results showed that the incision method was better than the aspiration method for efficiency of equine oocyte collection （ 83% vs 46.5% ） （ P 〈 0.05 ）. There were no significant differences in the maturation rate observed among the two different maturation media. The maturation rate of equine oocytes ma- tured in medium M199 was 41.7% for Cp oocytes and 64.7% for Ex oocytes; in medium DMEM/F12 it was 46.7% and 66.7% （P 〉 0.05 ）. The maturation rate of Ex oocytes were higher in M199 than DMEM/F12, but were not significantly difference（ P 〉 0. 05 ）. The MII stage Cp oocytes cultured in different culture media were activated by exposure to inomyein 5 min followed by an incubation for 4 h in 2 mM/mL 6-DMAP and 10 μg/mL CHX; the percentage of cleaved rate （P 〉 0.05） , it was 45% in M199 medium and 57.1% in DMEM/F12 medium.
出处
《四川动物》
CSCD
北大核心
2011年第1期16-20,F0002,共6页
Sichuan Journal of Zoology
基金
内蒙古农业大学校内《体细胞克隆马》项目资助
关键词
马卵母细胞
体外成熟
孤雌激活
equine oocytes
in vitro maturation
parthenogenetic activation
