摘要
目的:建立一种对菟丝子及其同属混淆药材进行分类鉴别的方法。方法:采用近红外漫反射光谱结合移动窗口偏最小二乘法(Moving-window Partial Least Square,mwPLS)波段选择进行主成分分析;近红外光谱分析结果与HPLC指纹图谱进行比对。结果:选择的最优波段为5998～6553 cm-1与7000～7556 cm-1;主成分分析结果3种不同菟丝子可以基本分开,分类正确率达93%;近红外光谱法的分析结果与HPLC指纹图谱法对菟丝子药材的鉴别结果一致。结论:采用近红外漫反射光谱结合mwPLS波段选择进行主成分分析,初步对菟丝子和其同属混淆药材南方菟丝子、金灯藤进行了鉴别研究。
Objective： To establish a method to classify and identify cuscuta and its mixed medicinal materials belonging to the same genus.Methods： A combination of near-infrared diffuse reflectance spectroscopy and mwPLS was applied for band selection and principal component analysis（PCA）;near-infrared spectroscopy results were compared with HPLC fingerprint.Results： The selected optimal bands were 5998～6553 cm-1 and 7000～7556 cm-1;PCA classified three different cuscuta with an accuracy rate of 93%;the results of near-infrared spectroscopy analysis and HPLC fingerprint were consistent.Conclusions： The use of near-infrared spectroscopy to identify Cuscuta chinensis,C.australis and C.japonica choisy is feasible.
出处
《药学与临床研究》
2010年第6期534-536,共3页
Pharmaceutical and Clinical Research
关键词
菟丝子
近红外光谱
PCA分析
Semen Cuscuta
Near-infrared Spectroscopy
Principal component analysis
