摘要
本文研究中华花龟的核型和NORs,其目的:为花龟属的分类,进化及探索其细胞遗传特性提供资料.方法:以外周血淋巴细胞为材料,以蒸气固定—空气干燥法制备染色体标本,常规方法分析核型.用一步染色法显示NORs.结果:2n=52,NF=76,核型模式为9+5+12,花龟的一对NORs位于A组No.7长臂.结论:中华花龟的核型和NORs均具有Bataguinae亚科龟类的保守性特点,就NORs分析。
The paper reported the karyotype and Ag NORs of Ocadia sinensis. Take aim at offer data of taxonomy, evolution and cell genet for Ocadia. This paper describes analysis on karyocytes and Ag NORs in chromosomal specimen of peripheral blood lymphocytes of O.sinensis prepared by the colchicine hypolonic air drying technique, using normal karyotypic analysis. The Ag NORs by a 1 step, simple Ag NOR staining method is revealled. The results indicate that the diploid number of O.sinensis is 2n=52, NF=76, karyotypic formula is 9+5+12. The Ag NORs is appeared of the long arm of 7th pair of chromosomes of group A. This species, like most other members of Bataguinae, possess more strong conservatism. Besides, Ag NORs homology for O.sinensis and Rhinoclemmys were observed.
出处
《安徽师范大学学报(自然科学版)》
CAS
1999年第1期44-46,共3页
Journal of Anhui Normal University(Natural Science)
基金
国家自然科学基金
安徽省自然科学基金