摘要
目的建立测定曲安奈德益康唑乳膏中两组分含量的高效液相色谱法。方法色谱柱为Sepax Technologies C8色谱柱(250mm×4.6mm,5μm),以己烷磺酸钠0.94g,加乙腈-异丙醇-水(140∶140∶720)磷酸调节pH为3.0作为流动相A,以取己烷磺酸钠0.94g,加甲醇-水(900∶100)磷酸调节pH为3.0作为流动相B递度洗脱;流速为1.0mL/m in;检测波长为227nm;柱温为40℃。结果醋酸曲安奈的的线性范围为0.01-0.08mg/mL(r=0.9999),最低检测浓度为0.3μg/mL,平均回收率为99.94%;硝酸益康唑的线性范围为0.1-0.8mg/mL(r=0.9999),最低检测浓度为0.001μg/mL,平均回收率为99.78%。结论本方法专属性好,回收率高,方法简便,结果准确可靠,可用于测定醋酸曲安奈德和硝酸益康唑德含量测定。
Objective To determine triamcinolone econazole cream in the two sub-levels using high performance liquid chromatography.Methods The column was Sepax Technologies C8 column（250mm×4.6mm,5μm）,to take hexane Sodium 0.94g,plus acetonitrile-isopropyl alcohol-water（140∶140∶720） adjusted with phosphoric acid pH3.0 as mobile phase A,to take hexane Sodium 0.94g,added methanol-water（900∶100） adjusted with phosphoric acid pH3.0 as the mobile phase B;a flow rate of 1.0mL/min;detection wavelength 227nm;column temperature was 40℃.Results Triamcinolone annai of the linear range of 0.01mg/mL-0.08mg/mL（r = 0.9999）,the minimum detectable concentration 0.3μg/mL,the average recovery was 99.94%;the linear range of econazole nitrate was 0.1mg/mL-0.8mg/mL（r=0.9999）,the minimum detectable concentration 0.001μg/mL,the average recovery was 99.78%.Conclusion The method is specific,high recovery rate for the determination of triamcinolone acetonide and moral determination of econazole nitrate method is simple,accurate and reliable.
出处
《湖北中医学院学报》
2010年第6期28-30,共3页
Journal of Hubei College of Traditional Chinese Medicine
