摘要
目的:了解骨髓增生异常综合征(MDS)和急性白血病患者骨髓间充质干细胞(mesenthymal stem cells,MSCs)的生物学性状是否存在病理改变。方法:获取12例MDS、16例急性髓细胞白血病(AML)患者和8例正常人的骨髓MSCs,在低血清培养液中扩增。流式细胞仪和免疫组织化学检测MSCs免疫表型,通过油红O和Von kossa染色来检测MSCs向脂肪和骨的分化。通过长期骨髓细胞培养和集落形成实验检测MSCs支持造血的功能。结果:MDS骨髓MSCs和正常人骨髓MSCs具有相似的细胞形态、增殖能力和免疫表型,而且都可以在体外诱导生成骨和脂肪细胞。但是MDS骨髓MSCs支持造血能力有所下降。AML骨髓MSCs在体外生长缓慢,很难传代培养,不能分化为脂肪和骨细胞,在体外不能支持造血。结论:MDS骨髓MSCs在支持造血方面存在病理改变;AML骨髓MSCs在增殖、分化和支持造血方面存在病理改变。
Objective：To study whether the biology characteristics of mesenthymal stem cells（MSCs） derived from bone marrow of patient with hematological diseasese were altered by disease state.Method：Bone marrow mononuclear cells from acute myelogenous leukemia（AML,n=16） and myelodysplastic syndromes（MDS,n= 12） were obtained and cultured in expanded medium.The immunophenotype of MSCs derived from different sources were investigated by FACS and immunohistochemistry.Osteocyte and adipocyte differentiation were detected by Von Kossa staining and Oil-red O staining.Moreover,hematopoiesis support in vitro was detected by long term bone marrow culture and the methylcellulose progenitor assay.Result：Our results showed that MSCs derived from MDS were similar to normal adult bone marrow derived MSCs in morphology,growth properties,surface epitopes,and differentiation ability in vitro.It was shown that MSCs derived from MDS showed discounted support hematopoiesis in long term culture.However,MSCs isolated from AML showed limited proliferation capacity,impaired differentiation and haematopoiesis support ability.Conclusion：MSCs derived from MDS bone marrow have pathological changes in support hematopoiesis in vitro,and MSCs derived from AML display pathological changes in proliferation,differentiation and support hematopoiesis.
出处
《临床血液学杂志》
CAS
2010年第6期671-674,共4页
Journal of Clinical Hematology
基金
国家自然科学基金资助项目(No:30801051)
