摘要
为了明确陕西省小麦禾谷镰刀菌混合群(Fusarium graminearum species complex)的遗传多样性,利用4对E coRⅠ和M seⅠ引物对来自陕西省19个区县的162株小麦禾谷镰刀菌菌株进行了AFLP扩增。结果表明,4对引物均能扩增出数量不等的多态性条带,最少的6条,最多的20条,大部分扩增片段在100～750bp之间。利用NTSYS-2.1软件聚类分析表明,不同地区禾谷镰刀菌可分为两大类群,即类群A和类群B。这两大类群的分化和地理来源有明确相关性,类群A主要分布在关中地区,类群B主要分布在陕南地区。初步判断可能与两个地区生态环境和小麦主栽品种差异有关。各类群内的菌系与地理位置间的关系较为复杂,一些菌系与地理来源存在明确关系,而个别菌系与地理来源间的关系尚不能完全明确。还需进一步研究以明确各菌株与地理来源之间的关系。
In order to reveal the genetic diversity of the Fusarium graminearum species complex in Shaanxi,162 strains collected from 19 regions of Shaanxi were amplified by AFLP with four pairs of primers（EcoRⅠ and MseⅠ）.The results showed that each primer amplified a different number of fragments between 100-750 bp,with the least of 6 and the most of 20.The cluster analysis suggested that the F.graminearum species complex from Shaanxi province could be classified into group A and group B.There were obvious relationship between group differentiation and geographic location.The group A was mainly distributed in the middle region,while the group B in the south region of Shaanxi.The ecological environments and varieties of wheat in these two areas might be the main reason of this division.However,the relationship between strains and geographic location was complicated.Some strains had a certain relationship to the geographic location but some did not.The further research for the relationship between strains and geographic location is needed.
出处
《植物病理学报》
CAS
CSCD
北大核心
2010年第6期615-621,共7页
Acta Phytopathologica Sinica
基金
国家"十一五"支撑计划项目(2006BAD08A05)
国家公益性行业科研专项(nyhyzx08-048)
高等学校学科创新引智计划(B07049)
