摘要
目的探讨转染NF-κB诱捕物寡核苷酸(decoy ODN)对肝脏缺血再灌注损伤的影响。方法制作20只肝脏缺血再灌注SD大鼠模型,无序ODN对照组、decoy ODN实验组各10只。阻断肝脏血流后,实验组行脂质体包裹的NF-κBdecoy ODN溶液门静脉注射转染,对照组行脂质体包裹的无序ODN门静脉注射。开放肝脏血流后3h取出肝脏,应用病理学、EMSA和RT-PCR分别检测移植肝脏病理改变、肝脏组织NF-κB转录活性和TNF-αmRNA表达情况。结果实验组肝脏损伤减轻,肝细胞NF-κB转录活性、TNF-α mRNA表达较对照组明显减小或减少(均P<0.01)。结论 NF-κBdecoy ODN可有效抑制肝脏细胞NF-κB的激活从而抑制肝脏缺血再灌注损伤。
Objective To investigate the effects of NF-κB decoy ODN transfection on the hepatic ischemia reperfusion injury.Methods The portal vein and hepatic artery were blocked 15 minutes in 20 SD rats,which were random divided evenly into 1 experimental group or 1 control group.The ischemia livers of experimental group were injected with the Lipofectamine 2000 mediated NF-κB decoy ODN solution.The liver were taken out 3 hours after reperfusion.The histopathologic changes,activity of NF-κB and expression of TNF-α mRNA of reperfused liver were determined by HE staining,EMSA and transcription polymerase chain reaction（PCR）.Results The histopathologic changes,NF-κB activity and TNF-α mRNA expression in NF-κB decoy ODN group decreased significantly compared with scamble ODN control（P0.01）.Conclusion Transfection of NF-κB decoy ODN with Lipofectamine 2000 can abate the NF-κB activation in reperfused liver and prevent hepatic ischemia reperfusion injury.
出处
《临床消化病杂志》
2010年第6期331-334,共4页
Chinese Journal of Clinical Gastroenterology
基金
湖北省自然科学基金资助项目(2007ABA227)
