摘要
目的:分离纯化并鉴定驴乳乳清中蛋白质组分,为进一步研究驴乳用于辅助治疗疾病,以及为作为人乳替代品提供参考。方法:采用DEAE-52离子交换层析和SephadexG-100凝胶层析分离纯化驴乳乳清中蛋白质组分,并通过十二烷基硫酸钠——聚丙烯酰胺凝胶电泳法和高效凝胶渗透色谱法对所纯化蛋白质进行鉴定。结果:与牛乳乳清中蛋白组分相对比,发现驴乳乳清中存在3种未知蛋白质,分子质量分别为32、70.1、72.2kD。结论:驴乳中除了含有与牛乳相似的营养成分外,还具有其他生物活性成分,它们可能是一些保护性蛋白,在机体的抗病机制方面起着重要作用。
Objective： To provide a theoretical basis for further exploring the exploitability of donkey milk as an adjuvant for disease treatment or as a substitute for human milk. Methods： The protein fractions of donkey whey were separated and purified by DEAE-52 anion-exchange chromatography and Sephadex G-100 gel-filtration chromatography. The purified protein fractions were identified by SDS-PAGE and high-performance gel permeation chromatography. Results： Three unknown proteins having molecular masses of 32, 70.1 kD and 72.2 kD were found to be present in donkey whey in comparison with the protein composition profile of cow＇s milk whey. Conclusion： Along with the similar nutritional components to those in cow＇s milk, other bioactive components were also contained in donkey＇s milk, which might be some protective proteins playing an important role in protecting the organism against diseases.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第23期44-48,共5页
Food Science
关键词
驴乳
蛋白质组分
分离
纯化
donkey＇s milk
protein fraction
separation
purification
