摘要
目的：观察雷公藤红素诱导人肥大细胞系（ＨＭＣ－１）细胞凋亡过程中细胞形态的变化，并探讨其意义。方法：在ＨＭＣ－１细胞培养体系中加入雷公藤红素，用光镜、电镜观察细胞的形态变化，用ＴＵＮＥＬ和流式细胞术（ＦＣＭ）检测细胞凋亡。结果：细胞在雷公藤红素作用下呈葵样改变：细胞母体居中，周边围以形成或正在形成的凋亡小体。葵样细胞（ｓｕｎｆｌｏｗｅｒ－ｌｉｋｅｃｅｌ，ＳＬＣ）对锥虫蓝拒染，可伴有核染色质浓缩和着边，药物作用２０ｍｉｎ即可形成，持续数小时后完全裂解为凋亡小体，或凋亡小体游离，残留细胞母体，最后崩溃；ＳＬＣ的形成具有时间和药物浓度依赖性；在８ｈ内与ＴＵＮＥＬ标记和碘化丙啶染色／ＦＣＭ检测的凋亡结果有显著相关性；ＳＬＣ清晰易辨，个体内和个体间计数重复性良好。结论：ＳＬＣ是凋亡细胞的特征性形态改变，可作为检测ＨＭＣ－１细胞凋亡的定性和定量指标。
Objective: Apoptosis of human mast cell line (HMC1) could be induced by tripterine, one of the
pure ingredients of tripterygium wilfordii hook. The paper is to explore the cell changes in
morphology of apoptotic cells.HZ Methods: On or after exposure to tripterine, cells were
observed with microscopy and electromicroscopy. Apoptosis was detected by flowcytometry
and TUNEL. Results: Sunflowerlike cell (SLC), cell surrounded by formed or forming apoptotic
bodies with chromatin condensed or not, were seen in the apoptotic process, which was negtive
to trypan blue dye. SLC could occur in 20 minutes after incubated with tripterine and survive for
several hours. The formation of SLC was at a time and dose dependent manner. The results of
apoptosis assayed by SLC count were significantly correlated with that mearsured by TUNEL or
PI/FCM in first 8 h. The SLC could easily be determined by microscopy in the condition of
culture. The results of SLC counted by inter or intraexaminer were consistent. Conclusion: The
results suggest that SLC is a characteristic morphologic change of apoptotic cells, and can be a
good indicator for HMC1 cell apoptosis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第5期273-276,F003,共5页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
