摘要
目的探讨鞘内注射加巴喷丁对切口痛大鼠吗啡镇痛效应的影响。方法雄性sD大鼠，体重250g～280g，取鞘内置管成功的大鼠48只，随机分为6组（n=8）：假手术组（S组）鞘内注射人工脑脊液（ACSF）10μl后，吸入1．4％异氟烷5min，不制备模型；切口痛组（IP组）、加巴喷丁50μg组（G组）、吗啡2．5μg组（M1组）和吗啡5Fg组（M2组）于制备切口痛模型前30min分别鞘内注射ACSF10f-I、加巴喷丁50μg、吗啡2．5μg和5μg；加巴喷丁50μg＋吗啡2．5μg组（G＋M1组）于制备模型前30min鞘内注射加巴喷丁50μg和吗啡2．5μg。模型制备后2h时测定术侧机械缩爪反射阈值（MWT）和热刺激缩爪反应潜伏期（TwL）。结果与S组比较，IP组、G组、M1组MWT降低，TWL缩短（P〈0．05），G＋M1组和M2组MWT和TWL差异无统计学意义（P〉0．05）；与IP组比较，G＋M1组和M2组大鼠MWT升高，TWL延长（P〈0．05），G组和M1组MWT和咧L差异无统计学意义（P〉0．05）；与G＋M1组比较，G组和M1组MWT降低，TWL缩短（P〈0．05）。结论鞘内注射加巴喷丁可增强吗啡对切口痛大鼠的镇痛效应。
Objective To investigate the effects of intrathecal （IT） gabapentin on the analgesic efficacy of morphine in a rat model of incisional pain. Methods Forty-eight male SD rats in which IT catheters were successly inserted according to the method described by Yaksh were randomly divided into 6 groups （ n = 8 each） ： Ⅰsham operation group, Ⅱ incisional pain group, Ⅲ GBP 50 μg group, Ⅳ morphine 2.5 μ group, V morphine 5 μg group, and ~ morphine 2.5μg ＋ gabapentin 50 μg group. In group Ⅰ , IT artificial cerebrospinal fluid （ACSF） 10 μl was injected and then 1.4% isoflurane was inhaled for 5 min. IT ACSF 10 μl, gabapentin 50 μg and morphine 2.5 and 5 μg were injected 30 rain before the establishement of the model in group Ⅱ -Ⅴ- respectively. Paw withdrawt threshold （PWT） to mechanical stimulation and paw withdrawal latency （PWL） to a thermal nociceptive stimulus were measured at 2 h after the establishement of the model. Results Compared with group Ⅰ , MWT was significantly decreased and TWL was significantly shortened in group Ⅱ , Ⅲ and Ⅳ （P 〈 0.05）, but no significant change in MWT and TWL was found in group Ⅴ and Ⅵ （P 〉 0.05） . Compared with group Ⅱ , MWT was significantly increased and TWL was significantly prolonged in group V and Ⅴ （ P 〈 0.05 ）, but no significant change in MWT and TWL was found in group Ⅲ and PC （ P 〉 0.05）. MWT was significantly decreased and TWL was significantly shortened in group Ⅲ and Ⅳ compared with group Ⅵ （ P 〈 0.05）. Conclusion IT gabapentin enhances the analgesic efficacy of morphine in a rat model of incisionaI pain.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第9期1042-1044,共3页
Chinese Journal of Anesthesiology
基金
江苏省级高校重点实验室开放课题资助项目（KJS06002）
关键词
环己酸类
吗啡
疼痛
手术后
注射
脊髓
Cyclohexanecarboxylic acids
Morphine
Pain, postoperative
Injections, spinal
