摘要
目的脊髓减压术是治疗急慢性脊髓压迫症的常用方法，但治疗机理尚不十分明了。我们通过免疫组化的方法以探讨脊髓前角神经元中胆碱乙酰化酶在神经功能恢复中的作用。方法采用免疫组化方法对大鼠脊髓压迫模型及减压后的脊髓前角细胞中乙酰胆碱转化酶（ＣｈＡＴ）的表达进行了经时变化的比较研究。结果，脊髓压迫可使脊髓前角神经细胞中的ＣｈＡＴ的表达受抑制。在这些ＣｈＡＴ表达受抑的细胞中部分可恢复其ＣｈＡＴ的表达。根据细胞恢复ＣｈＡＴ表达时的脊髓受压状态，这些细胞可分为无条件可恢复细胞和条件可恢复细胞。受压脊髓神经细胞中ＣｈＡＴ再表达与脊髓神经功能恢复密切相关。结论脊髓减压术是通过拯救脊髓内的条件可恢复细胞而起作用的。
Objective To explore the effect of cholineacetyl transferase(ChAT) in the ventral hornof spinal cord on the neuro-function recovery. Methods A comparison study was performed with immuno-cytochemical method to study the changes in ChAT expression in the ventral hom of spinal cord between thespinal cord compression and post-decompression models in rats. Results Spinal cord compression could in-hibit the ChAT expression in ventral horn of spinal cord, and some of the neuronal cells could re-express ChATactivity in neuronal soma. According to the condition of compression in spinal cord, these neuronal cells couldbe divided into conditional reversible neurons and non-conditional reversible neurons. Furthermore, there-expression of ChAT activity in neuronal cells was closely related to the neuronal functional recovery.Conclusion Spinal cord decompression is able to rescue the conditional reversible neurons from the restingstate.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1999年第2期95-98,I001,共5页
Chinese Journal of Orthopaedics
