摘要
利用RT-PCR和SMART RACE的方法从大黄鱼肝脏中克隆了PPARβ全长cDNA。该序列长3390bp,5′端非翻译区146bp,3′端非翻译区1711bp,开放阅读框1533bp,可编码一个由510个氨基酸组成的蛋白质,该蛋白质分子量为57.2kDa、等电点为6.46。氨基酸序列分析表明,PPARβ基因具有较高的保守性,大黄鱼PPARβ与花鲈、金头鲷、欧鲽、大西洋鲑、红鳍东方鲀、人、黑猩猩、牛、兔及小鼠等10个物种的同源性为72%~91%,其中与花鲈同源性最高,为91%。用RT-PCR法检测PPARβ基因的组织表达,结果表明该基因在大黄鱼肌肉、眼、脾、肾、肝、鳃、心、肠和脑等9个组织中均有表达,其中肝脏组织表达量最大,肌肉最少。
A full-length cDNA of the PPAR β in Larimichthys crocea was amplified by RT-PCR and SMART RACE method.The cDNA was 3390 bp in size with 146bp 5′-UTR,1711bp 3′-UTR and 1533bp ORF.It encoded a protein of 510 amino acids with a molecular weight of 57.2 kDa and pI 6.46.The sequence analysis indicated that the deduced amino acid sequence of the cDNA shared high identity with other ten species from 72% to 91%,and the highest identity was 91% with that of Lateolabrax japonicus.The RT-PCR analysis revealed that PPAR β mRNA was expressed in all tested tissues including the muscle,eye,spleen,kidney,liver,heart,gill,intestine and brain.Furthermore,the expression level of PPAR β was higher in liver than that in others.The lowest expression was in muscle.
出处
《生物学杂志》
CAS
CSCD
2010年第6期1-4,共4页
Journal of Biology
基金
国家自然科学基金(30671608)
宁波市自然科学基金(2006A610088)
关键词
大黄鱼
PPARβ
克隆
组织表达
Larimichthys crocea
PPAR β
clone
tissues expression
