摘要
本研究旨在分析HLA-A、-B、-DRB1低分辨位点相合的非亲缘关系造血干细胞供受者HLA-A、-B、-C、-DRB1和-DQB1高分辨匹配程度。采用PCR-SBT进行HLA-A、-B、-C、-DRB1和-DQB1位点高分辨检测。结果表明:在HLA-A、-B、-DRB1低分辨位点相合的274对非亲缘关系供受者标本中:①患者总数为166例,供者为274例。97例(58.43%)患者为1个供者,47例(28.31%)患者为2个供者。②HLA-A、-B、-C、-DRB1、-DQB1座位10个位点高分辨配合情况为6/10相合32对(11.68%),7/10相合为54对(19.71%),8/10相合为62对(22.63%),9/10相合为49对(17.88%),10/10相合为48对(17.52%)。③供受者HLA-A、-B、-C、-DRB1、-DQB1座位相合程度不同,以HLA-C位点不相合程度最高。274例供者检出23个HLA-A、46个HLA-B、21个HLA-C、30个HLA-DRB1和17个HLA-DQB1等位基因。166例患者检出20个HLA-A、40个HLA-B、22个HLA-C、29个HLA-DRB1和16个HLA-DQB1等位基因。④274例供者检出313条单倍型,166例患者检出224条单倍型,两者间频率最高的单倍型为A*02:07-B*46:01-C*01:02-DRB1*09:01:02-DQB1*03:03(5.63%和6.88%)。结论:在HLA-A、-B、-DRB1低分辨位点相合的基础上,非亲缘关系造血干细胞供受者HLA-A、-B、-C、-DRB1和-DQB1座位高分辨完全相合的概率较低。
This study was aimed to analyze the possibility of high resolution matching for human leukocyte antigen（HLA） loci in unrelated donor-recipient pair with low resolution match in HLA-A, -B, -DRB1 loci. Samples were genotyped for HLA-A, -B, -C, -DRB1 and -DQB1 by polymerase chain reaction sequence based typing （PCR-SBT）. The results showed that the total number of patients and the donors were 166 and 274. 97 （58.43%） patients were matched for 1 donor and 47 （28.31%） patients were matched for 2 donors at low resolution level; among 274 donor-recipient pairs, HLA-A, -B, -C, -DRB1 and -DQB1 loci matching for 6/10, 7/10, 8/10, 9/10 and 10/10 were 32 （11.68%）, 54 （19.71%）,62 （22.63%）,49 （17.88%） and 48 （17.52%） respectively; there were mismatch in HLA-A, -B, -C, -DRB1 and -DQB1 loci, and the most mismatch was in HLA-C locus. The number of alleles of HLA-A, -B, -C, -DRB1 and -DQB1 loci were 23,46,21,30 and 17 respectively in the donors. The alleles number HLA-A, -B, -C, -DRB1 and -DQB1 loci were 20,40,22,29 and 16 respectively in the patients; the haplotype number of HLA loci were 311 in the donors and 224 in the patients. The high frequency of haplotype was A02：07-B46：01-C01：02-DRB109：01：02-DQB103：03 （5.63% and 6.88%）. It is concluded that the probability of high resolution mismatch of HLA loci is high in unrelated donor-recipient pairs with low resolution match in HLA-A, -B, -DRB1 loci.
出处
《中国实验血液学杂志》
CAS
CSCD
2010年第6期1617-1620,共4页
Journal of Experimental Hematology
