摘要
为了明确1组在中温下(30℃)高效分解木质纤维素的复合菌系的菌群组成,研究复合菌系预处理秸秆对沼气发酵的影响,利用平板分离法和变性梯度凝胶电泳(DGGE)法研究了中温木质纤维素降解复合菌系BYND-8的菌种组成多样性,通过添加该复合系菌液到以牛粪为原料的沼气发酵体系,研究了添加秸秆降解液对沼气产量的影响.利用平板法分离得到了6株细菌,它们与Serratia sp.PSGB 13、Serratia marcescens strain UFLA-25LS、Serratia marcescens strain DAP33、Alcaligenes sp.YcX-20、Stenotrophomonas maltophilia strain C6和Bacillus cereus isolate BRL02-71的相似率分别达到了99%、100%、96%、100%、100%和99%.同时利用DGGE方法还检测到了1株利用平板分离法没有获得到的细菌,它的16S rDNA V3区的序列与Uncultured bacterium clone ATB-KS-1446具有100%的同源性.稻秆经复合菌系BYND-8预处理后应用于沼气发酵中,在发酵的前15d内,累积产气量达到13 167 mL,甲烷产量达到7 248 mL,比对照分别提高了44.5%和95.3%.复合菌系具有较高的菌种组成多样性,将复合菌系应用于沼气发酵的原料预处理过程中,可以将产气时间提前,并提高产气量.
The biodiversity of a mesophilic microbial community BYND-8 capable of degrading lignoeellulose at 30℃ was detected using denaturing gradient gel electrophoresis（DGGE） and the isolation of pure cultures, and the effect of the liquid of rice straw degradation by BYND-8 on biogas production was measured. Six bacterial strains were isolated using peptone cellulose solution medium, and the highest similarities of their 16S rDNA gene sequences to Serratia sp. PSGB 13, S. marcescens strain UFLA-25LS, S. marcescens strain DAP33, Alcaligenes sp. YcX-20, Stenotrophomonus maltophilia strain C6, Bacillus cereus isolate BRL02-71 were 99% , 100% , 96% , 100% , 100% and 99% , respectively. In addition, one band was detected besides six bands of cultured isolates on the DGGE gel, and it showed 100% sequence similarity to uncultured bacterium clone ATB-KS-1446. The cumulative biogas and methane productions of biogas fermentation system added with the liquid of rice straw degraded by BYND- 8 were 13 167 mL and 7 248 mL, 44. 5% and 95.3% higher than those of the control, respectively, in the early 15 days of fermentation. The results showed that the biodiversity of microbial community BYND-8 was very high, and the time of producing biogas was put forward and biogas production was increased with application of microbial community for rice straw pretreatment during the biogas fermentation.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2011年第1期253-258,共6页
Environmental Science
基金
"十一五"国家科技支撑计划项目(2008BADC4B01
2006BAD07A10
2006BAD04A09)
国家高技术研究发展计划(863)项目(2007AA100705)
黑龙江省"十一五"科技重点攻关项目(GB06B502
GA07B501
GB08B502
JC01B509)
黑龙江省普通高等学校新世纪优秀人才培养计划项目(1153-NCET-003)
黑龙江省普通高等学校青年学术骨干支持计划项目(1151G030)
博士后研究人员落户黑龙江科研启动项目(LBH-QO8O27)
黑龙江省杰出青年科学基金项目(JC201002)
关键词
木质纤维素降解
中温复合菌系
菌种组成多样性
变性梯度胶凝胶电泳
沼气发酵
lignocellulose degradation
mesophilie microbial community
bacterial diversity
denaturing gradient gel eleetrophoresis （DGGE）
biogas fermentation
