摘要
目的给予长效血管紧张素转换酶抑制剂（ＡＣＥＩ）———ｒａｍｉｐｒｉｌ后的大鼠，观察心肌梗塞（ＭＩ）后心功能以及心肌组织中血管紧张素Ⅱ（ＡｎｇⅡ）受体基因表达的改变。方法通过冠状动脉结扎建立大鼠ＭＩ模型，手术前１周即开始给予ｒａｍｉｐｒｉｌ或水，术后在存活大鼠中继续治疗至术后３小时、１日、３日、７日，每时间点大鼠６只；通过心室内插管测定心功能；用半定量ＲＴＰＣＲ法检测大鼠室间隔组织的ＡｎｇⅡ受体ｍＲＮＡ水平。结果（１）给予水的大鼠在ＭＩ后１日，其左心室舒张末期压（ＬＶＥＤＰ）明显增高，其心肌收缩功能在ＭＩ后３小时、１日、３日、７日均明显降低。（２）给予ｒａｍｉｐｒｉｌ的大鼠ＭＩ后１日、３日ＬＶＥＤＰ显著降低，而ｒａｍｉｐｒｉｌ改善心肌收缩功能的作用出现在ＭＩ后７日。（３）大鼠ＭＩ后室间隔组织中ＡｎｇⅡ受体ｍＲＮＡ水平升高，在３日时达到高峰。（４）给予ｒａｍｉｐｒｉｌ的大鼠ＭＩ后室间隔组织中ＡｎｇⅡ受体ｍＲＮＡ水平与用水的大鼠相比差异无显著性。结论给予ｒａｍｉｐｒｉｌ的大鼠ＭＩ后１周内的心功能明显改善，但大鼠主要心功能指标的改善不同步，提示ｒａｍｉｐｒｉｌ在ＭＩ后早期（１日，３日）和稍晚（７日）的作用及其作用?
Objective To investigate the early changes of cardiac function and ANG II receptor gene expression in myocardium after myocardial infarction in rats (n=6) treated with angiotensin converting enzyme inhibitor (ACEI)ramipril Methods Myocardial infarction (MI) was induced by left descending anterior coronary artery (LAD) ligation in rats (n=6) and shamoperated rats (n=6) were used as control Rats were treated with either longterm ACEIramipril or water Treatment was initiated 1 week before MI and continued till the day (3 hour; 1st, 3rd and 7th day after MI) of measurement Cardiac function parameters such as mean blood pressure (MBP), left ventricular enddiastolic pressure (LVEDP), left ventricular systolic pressure (LVSP), left ventricular contractility (LV dp/dtmax) were measured by arterial and intraventricular catheterization Semiquantitative RTPCR was applied to determine the AT1, AT2 receptor mRNA levels in the noninfarct myocardium Results (1) In watertreated rats, LVEDP increased one day after MI and myocardial contractile function decreased 3hour ,1st,3rd, and 7th day after MI (2) In ramipriltreated rats, LVEDP lowered significantly at 1st and 3rd day after MI, and the myocardial contractile function recovered at the 7th day after MI(3) Angiotensin receptor AT1 and AT2 mRNA level in the septum increased and reached a peak at 3rd day after MI (4) There was no significant difference in the myocardial AT1 and AT2 mRNA level between the ramipriltreated and watertreated rats after MI Conclusion In ramipriltreated myocardial infarction rats, the damaged myocardial function recovered significantly at the 7th day, however, the postinfarction changes in LVEDP and other myocardial contractility indices showed different time courses, possibly indicating different effects and mechanisms of ramipril in early and late postinfarction phases The AT1 and AT2 receptor gene expression in the noninfarct myocardium was possibly involved
出处
《中华心血管病杂志》
CSCD
北大核心
1999年第3期219-223,共5页
Chinese Journal of Cardiology
基金
卫生部项目基金
国家教委博士点基金
上海市曙光计划
关键词
心肌梗塞
ACEI
AngⅡ受体
逆转录PCR
myocardial infarctionangiotensin converting enzyme inhibitorAng receptor reverse transcriptional PCR
