摘要
目的探讨p5 3凋亡刺激蛋白基因(ASPP)的抑制蛋白iASPP在乳腺浸润性小叶癌中的表达及其临床意义。方法采用RT-PCR技术扩增乳腺浸润性小叶癌组织中iASPP mRNA;同时应用Quality One软件分析iASPP扩增产物的相对含量,分析iASPP-mRNA与临床病理因素之间的关系。结果 3 9例浸润性小叶癌组织中有3 4例表达iASPP(8 7.2%),5例不表达(1 2.8%);癌旁组织中均不表达iASPP。iASPP-mRNA在乳腺浸润性小叶癌中的表达明显增高,与癌旁组织相比差异具有显著性(P<0.0 1)。不同的年龄组iASPP-mRNA的表达差异也有显著性;TNMⅢ、Ⅳ期乳腺癌组织中iASPP-mRNA的表达显著高于Ⅰ、Ⅱ期患者(P<0.0 1);有淋巴结转移的癌组织中iASPP-mRNA的表达也高于无淋巴结转移者;iASPP-mRNA的表达与雌、孕激素受体的表达无关。结论 iASPP-mRNA在乳腺浸润性小叶癌中高表达。检测iASPP可以为乳腺癌的诊断、个体化治疗及预后提供参考。
Objective To study the level of inhibitory member of the ASPP family（iASPP） in invasive lobular breast carcinoma（ILBC） and its clinical significance.Methods The expression of iASPP-mRNA in the ILBC was analyzed by RT-PCR,then the average optional density of RT-PCR products was assayed by Quality one soft-ware.The relationship between the level of iASPP-mRNA and clinical pathological factors was analyzed.Results The iASPP-mRNA was expressed in 34 cases of ILBC（87.2%）,but there were no expression in adjacent tissues of the tumor.The expression of iASPP-mRNA in cancer tissues was markedly higher than that in adjacent tissues（P0.01）.There was significant difference between the patients aged≤45 years group and 45 years group（P0.01）,between TNM stage Ⅲ、Ⅳ cases and stage Ⅰ、Ⅱ cases（P0.01）,and between lymph node metastasis cases and none lymph node metastasis cases（P0.01）.There was no difference between the expression of iASPP-mRNA and the expression of estrogen receptor（ER） or progesterone receptor（PR）.Conclusions iASPP-mRNA is over-expressed in ILBC,and the detection of iASPP may be useful in the diagnosis,individual treatment and predicting the prognosis of ILBC.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2010年第11期1223-1226,共4页
China Journal of General Surgery
