摘要
【目的】研究重组人内皮抑素(recombinant human endostatin,rhES)能否抑制胃肠间质瘤(gastrointestinal stromaltumor,GIST)裸鼠移植肿瘤血管生成及生长,探讨抑制血管形成作为GIST治疗方法的可行性。【方法】随机将20只荷瘤裸鼠分为实验组与对照组。对照组瘤周注射生理盐水,实验组瘤周注射rhES,连用10d。自用药开始,每5d测量肿瘤体积,直至用药结束后5d。计算抑瘤率和肿瘤缩小率。比较实验组与对照组肿瘤体积、微血管密度(microvascular density,MVD)、bcl-2阳性表达率、凋亡指数(AI)及实验组治疗前后肿瘤体积差异是否具有显著性。【结果】用药前实验组与对照组肿瘤体积无差别(P=0.628)。用药后实验组肿瘤体积逐渐减小。用药结束后5d,实验组肿瘤体积与用药前及对照组相比均明显缩小(P=0.000),抑瘤率86.5%,肿瘤缩小率为55.5%。所有瘤株CD117均为阳性表达。实验组MVD低于对照组(P=0.020)。实验组bcl-2阳性表达率均低于对照组(P=0.023)。实验组AI高于对照组(P=0.020)。【结论】rhES抑制GIST血管生成、促进肿瘤细胞凋亡及遏制肿瘤生长,有可能成为治疗GIST的有效方法之一。
【Objective】 To investigate whether rhES can inhibit the angiogenesis and growth of GIST xenografts in nude mices,and the feasibility of anti-angiogenesis to become treatment method for GIST. 【Methods】 Twenty Balb / c-nu / nu mice burdened GIST were randomly divided into two groups. NS 0.1 mL in control group and rhES 2 mg / kg in experimental group were injected around the tumor,once a day for 10 days. The tumor bulk was measured every five days until five days after the end of test. The tumor inhibition rate （TIR） and tumor condensing rate （TCR） were calculated. The difference of tumor bulk,microvascular density （MVD）,bcl-2 positive expression rate and AI between the two groups were assessed. The tumor volumes between before and after treatment in experimental group were compared. 【Results】 The difference of the tumor bulk between the two group was no statistical significance before treatment （P = 0.628）,but at the end of test,the difference was significant （P = 0.000）,and in the test group the tumor bulk was also decreased significantly after treatment （P = 0.000）. In experimental group,the TIR and TCR were 86.5% and 55.5%,respectively. All xenografts showed CD117 positive staining. MVD and bcl-2 positive rate presented lower in experimental group than those in control group （P = 0.020 and P = 0.023,respectively）. AI increased significant in experimental group compared with in control group （P = 0.020）. 【Conclusions】 rhES can reduce angiogenesis,promote cell apoptosis,and inhibit the growth of GIST xenograft. It is possible that rhES becomes an effective drug for GIST in the future.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2010年第6期798-801,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省科技厅基金(2008B060600022)
教育部博士点新教师基金(20070558266)
广东省科委基金(2007B031515004)
