摘要
目的研究树突细胞特异性细胞间黏附分子一3结合非整合素因子（dendritic cells specific intercellular adhesion molecule-3-grabbing nonintegrin, DE-SIGN ）启动子区-139和-336位点多态性与HIV易感性、感染途径以及疾病进展的关系。方法比较160例HIV感染者和178名健康对照人群DC—SIGN启动子区-139位点和-336位点基因型分布的差异，采用Spearman秩和相关检验分析DC—SIGN启动子区-139和-336多态性与HIV感染的关系。结果在160例HIV感染者中，出现92例（57．5％）-139C和68例（42．5％）-139T，29例（18．1％）-336C和131例（81．9％）-336T。-139T／C和-336T／C基因型分布在HIV感染者与健康对照者中的差异无统计学意义（X2=0．121和1．754，P值均〉0．05）。通过性接触或静脉注射毒品而感染HIV的患者中，-139T／C和-336T／C分布差异无统计学意义（z2=0．435和0．103，P值均〉0．05）。在所有研究对象中，-139C基因型的个体多伴随-336C基因型（r=0．359，P〈0．01）。在外周血CD4＋T细胞计数≤50个／μL的患者中，-139T基因型出现的频率（27．9％）高于-139C基因型（23．0％）（x2=4．055，P〈0．05）。-139T／C和-336T／C对HIVRNA载量影响不明显（t=-0．643和-1．637，P值均〉0．05）。结论DC—SIGN启动子区-336C常与-139C耦联出现；-139或-336位点多态性与HIV的易感性和感染途径无明显相关性，但-139T基因型患者更易出现CD4＋T细胞数量的下降。
Objective To investigate the polymorphisms of -139 and -336 nucleotides in dendritic cells specific intercellular adhesion molecule-3-grabbing nonintegrin （DC-SIGN） promoter region in context of HIV susceptibility, infection routines and HIV/AIDS progress. Methods Polymorphisms of -139 and -336 nucleotides in DC-SIGN were examined in 160 HIV-positive subjects and 178 healthy controls; the Spearman test was performed to analyze their associations with HIV infection status. Results In 160 HIV- positive subjects, there were 92 （57.5%） with-139C, 68 （42.5%） with-139T, 29 （18.1%） with-336C and 131 （81.9%） with -336T. The frequencies of-139T/C and -336T/C in HIV-positive subjects were similar to those in the healthy controls （X2 = 0. 121 and 1. 754, P 〉 0.05 ）. No differences were found in the distribution of -139T/C or -336T/C in HIV-positive subjects infected via sex intercourse or intravenous drug （X2 = 0. 435 and 0. 103, P 〉 0.05）. -139C was usually eompanied with -336C （r = 0. 359, P 〈 0. 01 ）. -139T （27.9%） were more frequently presented in patients with CD4 ＋ T cells ≤50 cells/μL than -139C （23.0%, X2 = 4. 055, P 〈 0.05 ）. -139T/C and -336T/C were not related to HIV RNA levels （t = -0.643 and - 1. 637, P 〉 0.05）. Conclusions Genotype -139C in DC-SIGN promoter region usually coexist with -336C. Polymorphisms of -139 and -336 are not related to HIV susceptibilities or HIV infection routes. -139T genotype may be related to serious depletion on CD4 ＋T cells.
出处
《中华临床感染病杂志》
CAS
2010年第4期204-208,共5页
Chinese Journal of Clinical Infectious Diseases
基金
福建省自然科学基金（2008J0304）
福建省青年科研基金（2008-1-46）
