摘要
目的观察黄芩苷对动脉粥样硬化(AS)模型大鼠的凝血酶激活纤溶抑制物(TAFI)水平及血脂、凝血纤溶指标的影响,探讨黄芩苷在干预AS进程中的机制。方法 40只健康雄性W istar大鼠随机分为正常对照组、模型组、黄芩苷治疗组、辛伐他汀组,每组10只。正常对照组给予普通饲料喂养,其余3组在给予高脂饲料喂养的基础上行主动脉球囊损伤术,喂养4个月,以复制大鼠AS模型。用全自动生化仪测定血浆总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),用全自动血凝仪测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(F ib),发色底物法测定血浆TAFI的活性。结果 与正常对照组相比,其余各组大鼠的TC、TG、LDL-C、F ib和TAFI活性均明显升高(P<0.01或P<0.05),PT、APTT明显缩短(P<0.01或P<0.05);实验结束时,与模型组相比,黄芩苷治疗组与辛伐他汀组的TC、TG、LDL-C、F ib和TAFI活性明显低于模型组(P<0.01或P<0.05),PT、APTT明显延长(P<0.01或P<0.05);与辛伐他汀组比较,黄芩苷治疗组TC、TG、LDL-C、F ib、PT、APTT改善程度稍低(P<0.05),而TAFI活性则无明显统计学差异(P>0.05)。结论黄芩苷可能通过降血脂、改善凝血纤溶系统平衡、下调TAFI水平等途径干预了AS的形成。
Objective To investigate the effects and possible mechanisms of baicalin on the atherosclerosis（AS） rats.Methods 40 healthy Wistar rats were divided randomly into four groups： normal control group（n=10） and fed with normal diet,AS model group（n=10）,baicalin therapy group（n=10）,and positive control group（n=10）,rats in the last three groups were fed with hypercholesterol diet for 16 weeks.Treated with bacalin 100 mg· kg-1·d-1,and simvastatin 5 mg· kg-1·d-1 additionally for 16 weeks in baicalin therapy and positive control groups respectively from the second week.Serum lipids,blood coagulation,fibrinolysis and thrombin-activatable fibrinolysis inhibitor（TAFI） were detected with standard enzymatic assays.Results The levels of total cholesterol（TC）,triglycerides（TG）,low-density lipoprotein cholesterol（LDL-C）,prothrombin time（PT）, activated partial thromboplastin time（APTT）,fibrinogen（Fib） and TAFI in the three model groups were higher than those of normal control group（P〈0.01 or P〈0.05）.After treatment,the levels of serum lipids and Fib,TAFI in baicalin group were lower than those in non-drug treatment group obviously（P〈0.01 or P〈0.05）,and the levels of PT,APTT were longer too（P〈0.01 or P〈0.05）.Conclusions Baicalin improves AS not only by decreasing levels of serum lipids,the activity of blood clotting system and TAFI,also enhancing the activity of fibrinolysis system.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2010年第22期3299-3301,共3页
Chinese Journal of Gerontology
基金
山东省科技攻关计划项目(2006GG2202045)
关键词
动脉硬化
血脂
纤维蛋白原
凝血酶激活的纤溶抑制物
黄芩苷
Atherosclerosis
Serum lipids
Fibrinogen
Thrombin-activatable fibrinolysis inhibitor
Baicalin
