摘要
建立了加杨叶中芦丁含量测定的高效毛细管电泳方法。用甲醇溶解样品采用超声提取,研究了缓冲溶液浓度、pH值、电压、温度及进样时间等因素对分离检测的影响;确定了最佳的测定条件:50mmol/L硼酸盐缓冲溶液(pH值为9.3),压力进样时间为5s,温度为25℃,运行电压为20kV,检测波长为254nm。在此条件下,峰面积与芦丁浓度具有良好的线性关系,平均回收率达到98.58%,方法准确。同时,利用建立的方法研究了加杨叶生长过程中芦丁含量的变化。
The method for determining rutin content of Populus × canadensis Moench. leaves was developed by high performance capillary electrophoresis（HPCE）. Sample was extracted with methanol by ultrasonic. The optimum determination conditions were investigated experimentally and the results were as follows： the running buffer of 50 mmol/L borate （pH = 9.3）, injection time with pressure of 5 s,temperature of 25℃ ,applied voltage of 20 kV,detection wavelength of 254 nm. Under the optimum conditions, a good linearity between peak area and concentration of rutin was found. The average recovery is 98.58% and the method is accurate. Meanwhile, the developed method was utilized to investigate the variation of rutin content during the growth of P. × canadensis leaves.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2010年第6期9-12,共4页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(30671655)
关键词
高效毛细管电泳
加杨叶
芦丁
high performance capillary electrophoresis
Populus × canadensis Moench. leaves
rutin
