摘要
分别以海藻酸钠、壳聚糖复合Fe3O4为载体,采用包埋―交联法固定β–呋喃果糖苷酶。对固定化过程氯化钙浓度、戊二醛浓度、加酶量、包埋时间、交联时间等因素进行考察;并采用正交试验对载体制备与酶固定化中主要条件进行优化;通过对固定化酶活力回收比较,磁性海藻酸钠固定化酶活力回收率优于磁性壳聚糖。
β-fructofuranosidase was co-immobilized by embedding-crosslinking with sodium alginate and chitosan complexed with Fe3O4.Some factors that have effect on activity of immobilized β-fructofuranosidase were studied.Such as the concentration of Calcium chloride,the density of glutaraldehyde,amount of β-fructofuranosidase,embedding time,crosslinking time and so on.The main conditions were finally optimized and determinated by the orthogonal experiments.Magnetic sodium alginate is better than magnetic chitosan,by comparing of yield of immobilized enzyme
出处
《粮食与油脂》
北大核心
2010年第11期21-24,共4页
Cereals & Oils
基金
陕西省教育厅基金项目(08JK238)
