摘要
目的:观察附子理中丸对脾阳虚证大鼠骨骼肌能荷的影响。方法:18窝新生大鼠按同窝匹配、体重均衡原则随机分9组,前5组不切除甲状腺作为获得性脾阳虚,后4组切除甲状腺作为发育性脾阳虚。d_1切除部分甲状腺。d_(42)切除肩胛骨间棕色脂肪,d_(56)高脂饲养和d_(70)用药防治。d_(98)取骨骼肌,高效液相色谱测量能荷。t检验比较组间效能差别。结果:获得性脾阳虚大鼠中,阳虚组能荷值比正常组降低14.2%(P<0.01),脾阳虚组能荷值比阳虚组降低16.0%(P<0.01),凉治组能荷值比脾阳虚组升高5.8%(P<0.01),温治组能荷值比脾阳虚组降低9.6%(P<0.01)。发育性脾阳虚大鼠中,阳虚组能荷值比正常组升高45.6%(P<0.01),脾阳虚组能荷值比阳虚组降低23.9%(P<0.01),凉治组能荷值比脾阳虚组升高6.8%(P<0.01),温治组能荷值比脾阳虚组降低16.8%(P<0.01)。与获得性脾阳虚比较,发育性脾阳虚阳虚组、脾阳虚组、凉治组和温治组的能荷值分别升高69.7%、53.8%、55.4%和41.7%(P<0.01)。结论:降低骨骼肌能荷是附子理中丸增强发育性和获得性脾阳虚大鼠产热潜能的机制之一。
Objective： To observe the effects of aconitum lizhong pills on the levels of energy charge in the skeletal muscle of rats with spleen yang deficiency syndrome. Method： Eighteen littermates, each litter was bred sequentially and random divided into 9 groups. Five groups were not carried out thyroidectomy, the other four groups did. Thyroidectomy in hypothyroidism littermates were carried out with ^131 iodine to destroy partly thyroidal tissue on d1 after birth,the interscapular brown adipose tissue was removed on d42,littermates were fed with more cholesterol ester and triglyceride from d56, then administered intragastrically a decoction of medical herbs to promote or inhibit chronic thermogenesis from d70. The levels of energy charge in skeletal muscle was quantified by HPLC at d98. The potency data derives from the primary data. A t-test was used to evaluate the change between groups,P 〈 0. 05 meaned statistic significance. Result： In the euthyroid rats, compared with the control group, the levels of energy charge in skeletal muscle was decreased by 14. 2% （P 〈0.01 ） in model rats （rats with yang deficiency syndrome）. The level in fat-diet rats （rats with spleen yang deficiency syndrome） was decreased 16. 0% （P 〈 0. 01 ） than that in the model rats. The rats treated with huanglian jiedu decoction increased the levels by 5.8% （P 〈0.01 ） and the rats treated with aconitum lizhong pills decreased by 9. 6% （P 〈0. 01 ） than that in the fat-diet rats. In hypothyroid rats, compared with the control group, the levels of energy charge in skeletal muscle increased by 45.6% （ P 〈 0.01 ） in model rats;the level in fat-diet rats decreased 23.9% （P 〈0. 01 ） than that in the model rats;the rats treated with huanglian jiedu decoction increased the levels by 6. 8% （P 〈 0. 01 ） and the rats treated with aconitum lizhong pills decreased by 16. 8% （ P 〈 0.01 ） than that in the fat-diet rats. The levels potency of energy charge in the groups with hypothyroid were higher
出处
《中国药师》
CAS
2010年第12期1691-1694,共4页
China Pharmacist
基金
国家重大基础研究(编号:G2000056905)
国家自然科学基金(编号:30873464)
教育部重点项目(编号:108019)
关键词
附子理中丸
脾阳虚证
大鼠
骨骼肌
能荷
Aconitum lizhong pills
Spleen yang deficiency syndrome
Rat
Skeletal muscle
Energy charge
