摘要
目的研究STAT3信号传导通路对HepG2细胞p44/42MAPK蛋白表达和细胞生长的影响。方法将针对STAT3的siRNA转染入HepG2细胞以沉默STAT3基因的表达,采用MTT法检测细胞生长,采用Western blot法检测STAT3和p44/42MAPK蛋白的表达。结果对照组和lipofectamineTM2000处理组细胞生长无明显差异(q=0.97,P>0.05),而siRNA处理组细胞生长被明显抑制(q=9.36,P<0.05);SiRNA转染后24h、48h、72h和96h,细胞抑制率分别为33.2%、39.6%4、3.1%和33.9%s,iRNA在96h后抑制作用减弱,细胞开始重新繁殖;转染细胞72h和96h后,可见STAT3蛋白表达均被抑制(t=14.12,P<0.05),p44/42MAPK蛋白表达未被抑制(F=3.99,P>0.05),而p-p44/42MAPK蛋白表达增加(t=16.30,P<0.05)。结论 STAT3信号传导通路可以影响细胞生长及p44/42MAPK蛋白质的磷酸化,而p-p44/42MAPK的表达增加可能代偿了沉默STAT3引起的细胞生长抑制,使细胞重新繁殖。
Objective To study the effect of STAT3 signaling pathways on p44/42MAPK and cell growth in hepatocellular carcinoma.Methods The siRNA directed against STAT3 was transfected into HepG2 cells.Cell growth was measured by MTT assay and STAT3 and p44/42MAPK protein expression were measured by Western blot.Results A significant decrease in the number of viable cells（q=9.36,P0.05）was found in the siRNA transfection group as compared to that in the control or the LipofectamineTM2000 transfected groups;SiRNA inhibited cell growth with the inhibition rates of 33.2% at 24h,39.6% at 48h,43.1% at 72h,and 33.9% at 96h;At 72h and 96h after the transfection,STAT3 protein expression was inhibited and P44/42MAPK protein expression was not influenced and p-p44/42MAPK protein expression was increased.Conclusion siRNA against STAT3 may affect cell growth in vitro.
出处
《实用肝脏病杂志》
CAS
2010年第6期401-403,共3页
Journal of Practical Hepatology
基金
黑龙江省教育厅基金资助项目(编号:11531190)
哈尔滨医科大学附属第二医院青年基金资助项目(编号:QN2007-17)
