摘要
目的建立高效液相-质谱联用（LC/MS/MS）法测定人血浆盐酸伪麻黄碱浓度的方法。方法采用Thermo Hypurity C18色谱柱（150mm×2.1mm,5μm）;流动相：5mmol.L-1醋酸铵-甲醇（70：30）;流速：0.3mL.min-1;柱温：40℃;MRM模式检测（离子对）,内标法定量。结果血浆盐酸伪麻黄碱在17.67~565.30ng.mL-1浓度范围内线性关系良好（r=0.9995）。低、中、高3种浓度相对回收率分别为101.96%,102.08%,98.85%,日内、日间RSD均〈10%。结论该方法快速简便、灵敏准确,可用于盐酸伪麻黄碱血浆浓度的测定及其药动学研究。
Objective To establish a LC/MS/MS method for determination of pseudoephedrine hydrochloride in human plasma.Methods The analysis was carried out by using Thermo Hypurity C18 column（150 mm× 2.1 mm,5 μm）.The mobile phase consisted of 5 mmol·L-1 ammonium acetate-methanol（70：30）with a flow rate at 0.3 mL·min-1.The column temperature was set at 40 ℃ and concentrations in plasma were determined at ion pairs（166.2/148.2）by MRM of LC/MS/MS.Results The linear range of pseudoephedrine hydrochloride was 17.67～565.3 ng·mL-1（r=0.999 5）,the recoveries were 101.96%,102.08%,98.85%,at low,middle and high concentrations,respectively,and RSD were below 10%.Conclusion The method is simple and precise,which can be used for determination of pseudoephedrine hydrochloride in human plasma and its pharmacokinetics study.
出处
《医药导报》
CAS
2010年第12期1563-1565,共3页
Herald of Medicine
