摘要
[目的]探索一种分离纯化嗜热链球菌胞外多糖的途径。[方法]采用嗜热链球菌为材料,以MRS培养基为基础培养基,生产嗜热链球菌胞外多糖。采用酶法、TCA法、Sevag法、酶+TCA法、酶+Sevag法、TCA+Sevag法、酶+TCA+Sevag法研究对嗜热链球菌胞外多糖去蛋白的影响,对得到的粗多糖用DEAE-Cellulose离子交换柱和葡聚糖G-100凝胶柱进行了分离,并用紫外光谱扫描对其的纯度进行鉴定。[结果]粗多糖用蒸馏水和0.1 mol/L NaC l通过DEAE-Cellulose离子交换柱可以分为中性多糖和酸性多糖,用葡聚糖G-100凝胶柱对酸性多糖的纯度进行了鉴定,得到单一的峰,紫外光谱扫描对酸性多糖进行全波长扫描,酸性多糖中不含蛋白质和核酸,证明得到的酸性多糖为纯多糖。[结论]酶+Sevag法为最佳去蛋白法。
[Objective]To explore a way of isolating extracellularpoly saccharide from Streptococcus thermophilus.[Method]With Streptococcus thermophilus cultured on the MRS-based medium producted the exopolysaccharide（EPS） preparation.By enzymatic,TCA method,sevag,enzyme＋TCA,enzyme＋sevag,TCA＋sevag,enzyme＋TCA＋sevag methods were used to remove proteins form EPS preparation.EPS preparation was characterized using DEAE-Cellulose ion-exchange chromatography and dextran G-100 gel filtration.UV detection method were used to identify the purity of EPS.[Result]Crude EPS with distilled water and 0.1 mol/L NaCl through the DEAE-Cellulose ion-exchange column could be divided into neutral EPS and acidic EPS.Using dextran G-100 gel filtration on the purity of acidic EPS was identified by a single peak.Acidic EPS was wide wavelength scanned by UV spectrum scanning,acidic EPS did not contain proteins and nucleic acids,it proved acidic EPS is pure EPS.[Conclusion] The enzyme＋sevag method to remove proteins as the best method.
出处
《安徽农业科学》
CAS
北大核心
2010年第32期18019-18020,18023,共3页
Journal of Anhui Agricultural Sciences
基金
山西省教育厅项目(20091002)
关键词
嗜热链球菌
胞外多糖
纯化
Streptococcus thermophilus
EPS
Purification
