摘要
基于睾丸酮丛毛单胞菌(C.test)具有降解甾体类物质的特性,其细胞中3α-羟基类固醇脱氢酶/碳酰基还原酶(3α-HSD)是诱导酶,建立了以3α-HSD的测定总甾体激素含量的双抗夹心酶联免疫反应(ELISA)方法。实验结果表明,睾丸酮类甾体激素的量在0.5～600μg/g之间,与3α-HSD酶表达量具有明显线性关系。应用这种方法对多种水资源、饲料、肉食品进行了检测。检测结果表明,松花湖水中的睾丸酮类甾体激素含量为34.5μg/g,明显高于其它水系;被测饲料中睾丸酮类甾体激素含量均大于2μg/g;肉类样品的检测结果均低于检测下限。本方法与高效液相色谱法的测定结果相符。
Comamonas testosteroni secretes 3α-HSD/CR when induced by steroid hormones,then it has the characteristics to degrade steroid hormones.This work developed a Enzyme-linked immunosorbent assay（ELISA） method to detect the steroidal hormones in water,food and animal feed.The results show that the content of testosterone and 3α-HSD enzyme expression have a linear relationship.The linear range is within 0.5～600 μg/L.Adopting this method,the study detect the steroidal hormones in water,food and feed.Content of testosterone in Songhua Lake is 34.5 μg/L,which is higher than others.Content of testosterone in the 8 animal feed is more than 2 μg/L.Meat sample test shows that the amount of steroidal hormones is below the detection limit.This result from this method is consistent with that from HPLC.
出处
《应用化学》
CAS
CSCD
北大核心
2010年第12期1466-1469,共4页
Chinese Journal of Applied Chemistry
基金
吉林省科技厅重大项目(20096012)资助
