摘要
应用分散液相微萃取(DLLME)技术,建立了水中丙溴磷农药的高效液相色谱(HPLC)分析方法。考察了萃取剂、分散剂、萃取剂体积、分散剂体积、时间、盐度和pH等因素对分散液相微萃取的影响,并确定了最佳萃取条件为:15μL三氯乙烷(萃取剂)和700μL乙腈(分散剂),混匀后,加入水样,室温静置2min,以3000r/min离心2min,吸取3μL沉积相,进行HPLC分析。在此优化条件下,富集倍数达到270,检出限为2μg/L,相对标准偏差(RSD)为1.4%～6.1%(n=6);标准加入回收率为81.9%～118%。本方法操作简单,成本低,结果令人满意。
A method was developed for the determination of profenofos in water by dispersive liquid-liquid microextraction（DLLME）-high performance liquid chromatography（HPLC）.Some experimental parameters that influence extraction efficiency,such as the kind and volume of disperser solvents and extraction solvents,extraction time,addition of salt and PH,were investigated.The optimal experimental conditions were as follows：approximately 15μL of trichloroethane（extraction solvents）and 750μL of acetonitrile（disperser solvents）were added to the water samples,then set at room temperature for 2 min,centrifugation at 3000 r/min for 2 min.Finally,3μL of sedimented phase was injected into the HPLC for analysis.Under the optimum conditions,the enrichment factors of the profenofos was about 270,the detection limits was 0.002 mg/L.The recoveries for standard addition fell in the range from 86.9%to 118%and the relative standard deviations（RSD）were between 1.4%and 6.1%.The obtained results showed that the DLLME-HPLC method simple and low-cost.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第4期551-554,共4页
Chinese Journal of Analytical Chemistry
基金
江苏省环境科学与工程重点实验室项目(No.ZD071203)
江苏省环境工程重点学科建设基础研究项目(No.S10332083002)资助
关键词
分散液相微萃取
高效液相色谱
丙溴磷
水样
Dispersive liquid-liquid microextraction
High performance liquid chromatography
Profenofos
Water sample
