摘要
为了探讨非侵蚀性葡萄胎（ＨＭ）转化为侵蚀性葡萄胎（ＩＨＭ）的细胞生物学机制，本研究采用免疫组织化学法观察了ＮＧＦ和ＴＧＦ－β１在ＨＭ和ＩＨＭ中的分布。结果显示：在ＨＭ绒毛中，合体滋养细胞（ＳＴ）和新生基质细胞ＮＧＦ和ＴＧＦ－β１呈强阳性和阳性；老化基质细胞和细胞滋养细胞（ＣＴ）呈阴性；在ＨＭ的中间型滋养细胞，ＮＧＦ呈强阳性着色，ＴＧＦ－β１阴性；在ＨＭ绒毛干中，ＳＴ的ＮＧＦ呈强阳性，ＮＧＦ和ＴＧＦ－β１呈弱阳性；ＣＴ的ＮＧＦ呈弱阳性，ＮＧＦ和ＴＧＦ－β１为阴性；在ＨＭ的绒毛干近滋养层基质细胞，ＮＧＦ呈弱阳性，ＮＧＦ和ＴＧＦ－β１呈阳性；在远滋养层基质细胞，免疫组化染色皆为阴性。在ＩＨＭ的绒毛ＳＴ和ＣＴ免疫组化染色皆为阴性，在绒毛新生基质细胞，免疫组化染色呈阳性；在绒毛老化基质细胞。
In order to study the regulating mechanism of NGF and TGF-β 1 in HM and IHM,ABC immunohistochemistry method was used.The results show: In villi of HM,NGF and TGF-β 1 strongly positive and positive in new stromal cells and ST;old stromal cells of villi and villous cytotrophoblast megative; IT,NGF strongly positive,TGF-β 1 showing negative;in villous columm(VC)of HM,NGF strongly positive, TGF-β 1 showing negative,in villous column(VC)of HM,NGF strongly positive,TGF-β 1 weekly positive in ST,NGF weekly positive,TGF-B negative in CT;in stromal cells of proximal VC,NGF showing wddkly positive, TGF-β 1 positive; stromal cells of distal VC showing megative.In villous new stromal cells of IHM,NGF-β 1 positive; In villous old stromal cells, villous ST and CT of IHM,all negativt.
出处
《中国优生与遗传杂志》
1999年第2期21-22,15,共3页
Chinese Journal of Birth Health & Heredity
