摘要
用抗人T细胞单克隆抗体OKT_3、OKT_4,OKT_8检测急性多发性神经根神经炎(AP)患者T淋巴细胞细胞亚群。共检测AP患者23例,正常对照20例,并观察不同病情、病型患者的T细胞亚群的改变。结果表明AP患者OKT_a^+、KT_4^+细胞百分率与正常对照组相同,但OKT_8^+细胞百分率明显低于对照组(P<0.05),OKT_4^+/OKT_a^+细胞比率相应升高(P<O.05)。不同病情及病型患者间T细胞亚群无明显差异,但随病程延长OKT_a^+细胞和OKT_4^+/OKT_8^+细胞比率逐渐恢复正常。结果提示细胞免疫紊乱可能在AP发病中有重要作用。
Ficol-Hypaque separated peripheral bloo dmononuclear cells were analysed by indirect immunofluorescence assay with ortho monoclonal antibodies to T cells (OKT_3^+), helper cells (OKT_4^+) and suppressor cytotoxic cells (OKT_8^+). 20 normal subjects and 23 patients with acute polyradiculoneuritis (AP) wore studied, The percentages of OKT_3^+ and OKT_4^+ cells were similar in the 2 groups, but the percentages of OKT_8^+ cells was reduced to 18.96±4.24 compared with 22.28±3.15 in the the normal subjects (P<0.05). The ratio of OKT_4^+/OKT_8^+ cells was correspondingly increased to 2.38±0.55, compared with 1.97±0.20 in the normal subjects. These changes fell towards normal with the passage of time. The result suggests that AP might be an autoimmunity disease with cell immunity disorders.
关键词
神经根神经炎
T细胞亚群
acute polyradiculoneuritis
human T lymphocyte
monoclonal antibodies
indirect immunofluorescence assay
