摘要
[目的]提取高质量的山药叶片总RNA。[方法]以山药叶片为材料,采用异硫氰酸胍法、改良CTAB法提取山药叶片总RNA并进行比较分析,利用琼脂糖凝胶电泳分析所提RNA的完整性,并用紫外分光光度计分析RNA的纯度。[结果]改良CTAB法提取的总RNA的28S、18SrRNA条带清晰、整齐,测得A260/A280为1.72.0。[结论]改良的CTAB法适合山药叶片总RNA提取。
[Objective]The aim was to obtain high-quality total RNA from leaves of Dioscorea opposita.[Method]The guanidinium isothiocyanate method and modified CTAB method was used to extract total RNA from leaves of Dioscorea opposita respectively.By employing agarose gel electrophoresis and UV-spectrophotometer,the integrity and purity of total RNA were analyzed.[Result]The 28S and 18S bands of total RNA extracted by the modified CTAB method were clear and visible,and the A260/A280 value of it was from 1.7 to 2.0.[Conclusion]Modified CTAB method was suitable for the extraction of total RNA from Dioscorea opposita leaves.
出处
《安徽农业科学》
CAS
北大核心
2010年第33期18728-18729,共2页
Journal of Anhui Agricultural Sciences
基金
陕西理工学院引进人才科研启动项目(SLGQD0715)
陕西省教育厅专项科研项目(08JK246)
关键词
山药叶片
总RNA提取
改良CTAB法
异硫氢酸胍法
Dioscorea opposita leaves
Extraction of total RNA
Modified CTAB method
Guanidinium isothiocyanate method
