摘要
对全国分布区12个天然油松种群进行取样,测定了叶绿体DNAtrnT-trnL、trnS-trnG、trnL-trnF基因间隔序列,分析了序列碱基组成特点及在松科系统分类中的意义.测序结果显示,3个片段碱基序列均富含A/T,长度依次为448bp、636bp和421bp,所测片段在种群水平上十分保守,没有发现具有种内鉴别意义的碱基变异.但此片段适合于松科属间和种间的系统学分析,运用PHYLIP软件构建松科植物的邻接系统树,支持松科分为两个亚科的分类系统,拓扑结构表明松属与银杉属的关系最近.由于叶绿体DNA序列长度适中,扩增和测序较为容易,适合作为研究松科植物系统进化较为理想的分子标记.
The samples of Pinus tabulaeformis were collected from 12 populations occurring in whole China within its chloroplast DNA distribution range, and their noncoding sequences of chloroplast DNA trnT-trnL, trnS-trnG and trnL-trnF were sequenced. The characteristics of base compositions and the significance of the sequences in taxonomy of Pinaceae were analyzed by using the sequence data. The results indicated： （1） The three cpDNA sequences, 448 bp, 636 bp and 421 bp length, were characterized with a high A＋T content; and （2） the alignments of the three cpDNA sequences in P. tabulaeformis were identical and conservative, suggesting that the DNA sequences were not suitable for analyzing the intraspecific relationship of P. tabulaeformis, but they were found useful for analyzing interspecific and intergeneric relationships within the family. The NJ （neighbour-joining） tree constructed by using PHYLIP software package supported that Pinaceae could be divided into two subfamilies, revealing the closer relationship between Pinus and Cathaya. Because of their moderate length and easy amplification, the three DNA sequences were considered as ideal molecular markers to study the evolutionary systematics of Pinaceae. Fig 2, Tab 2, Ref 24
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2010年第2期143-147,共5页
Chinese Journal of Applied and Environmental Biology
基金
山西省回国留学人员科研项目(No.200846)
国家自然科学基金项目(No.30770294)资助~~
关键词
油松
cpDNA间隔序列
系统进化
分子标记
Pinus tabulaeformis
cpDNA spacer sequence
systematic evolution
molecular marker
