摘要
目的通过观察吸烟小鼠呼吸道上皮细胞上皮钙粘附素（Ｅｃｄ）表达的动态变化，探讨吸烟对呼吸道上皮细胞Ｅｃｄ表达的影响。方法应用免疫组织化学ＳＰ法，检测不同剂量、不同阶段吸烟小鼠呼吸道上皮细胞Ｅｃｄ的表达，并在光镜及电镜下观察其形态学变化。结果小鼠吸烟４周、１２周时，上皮细胞Ｅｃｄ表达与正常对照组比较明显下调（Ｐ＜００１），吸烟剂量加倍时其下调更为显著；形态学研究表明吸烟４周及１２周时上皮细胞间连接松散，上皮细胞损伤同时伴有增殖。吸烟８周时，Ｅｃｄ表达与正常对照组之间差异无显著性（Ｐ＞００５）。结论吸烟引起呼吸道上皮细胞Ｅｃｄ表达波浪式变化在呼吸道上皮细胞的损伤与修复中可能发挥重要作用。
Objective To investigate effects of smoking on Ecadherin (Ecd) expression of injury and repair of respiratory tract epithelium by observation of dynamic expression of Ecd on epithelial cells of respiratory tract in smoking mice. Methods Immunohistochemistry SP technique was used to examine Ecd expression on epithelial cells of respiratory tract in smoking mice of different stages and dosages and the morphological changes observed by light and electron microscopy respectively. Results Four and twelve weeks after smoking, the Ecd expression in the epithelial cells was significantly downregulated compared with the control group (P<001). Morphologically, the intercellular junction was loose, and the epithelial cells were injured and proliferated on the same time . Eight weeks after smoking, however, the Ecd expression was not different from that of the control group (P>005). Conclusions Smoking is able to cause wavy change of the Ecd expression on the epithelial cells which may play an important role in the injury and repair of the epithelial cell of the respiratory tract.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1999年第7期417-419,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家自然科学基金
