摘要
目的探讨长时间输注异丙酚对兔肝脏线粒体呼吸功能的影响。方法 18只成年新西兰兔随机分为3组:空白对照组、异丙酚组和脂肪乳组。空白对照组持续输注0.9%生理盐水,异丙酚组持续输注1%异丙酚,脂肪乳组持续输注10%脂肪乳。用差速离心法分离肝脏线粒体。用酶活性分析试剂盒测量肉碱脂酰转移酶(CPT)活性,定磷试剂法测量H+-ATP酶的分解活性,高效液相色谱法测量线粒体内三磷酸腺苷酸(ATP)含量。结果异丙酚组CPT活性较空白组降低(P<0.05),脂肪乳组和空白组比较差异无统计学意义(P>0.05)。异丙酚组H+-ATP酶的分解活性较空白组升高(P<0.05),脂肪乳组和空白组比较差异亦无统计学意义(P>0.05)。脂肪乳组和异丙酚组线粒体内ATP含量与空白组比较差异均统计学意义(P>0.05)。结论异丙酚可能通过干扰线粒体脂肪酸β-氧化影响细胞的能量代谢;脂肪乳作为异丙酚的脂质溶媒对线粒体呼吸功能无明显影响。
Objective To investigate the changes of respiratory function of liver mitoehondria in rabbits induced by the general anesthetic propofol. Methods Eighteen New Zealand rabbits weighted 1.5-2. 5 kg were randomly divided into three groups： control group, emulsion group and propofol group. The control group received continuous infusion of 0. 9% sodium ehloride solution. The propofol group received continuous infusion of 1% propofol. The emulsion group received continuous infusion of 10% emulsion. The liver mitochondri of the rabbits were isolated. The earnitine aeyl transferase （CPT） activity, H^＋-ATPase hydrolysis activity and the content of ATP in the mitoehondria were analysed. Results The rabbits in the propofol group had lower activity of CPT than the controls （P〈0. 05）, while no difference was found between the control group and the emulsion group （P〈 0. 05）. The rabbits in the propofol group had higher H^＋-ATPase hydrolysis activity than the controls （P〈0.05）, while no difference was found between the control group and the emulsion group （P〈 0. 05）. No significant differences were found in the eontent of ATP in mitoehondria between the three groups （P〉0. 05）. Conclusion Propofol inhibits CPT activity, which disturbs fatty acid β- oxidation. Emulsion acted as vehicle of propofol seems to have no significant impact on mitoehondria respiratory function.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2010年第6期1021-1023,共3页
Journal of Sichuan University(Medical Sciences)
