摘要
从猴头菌子实体中分离得到一种新型的水溶性杂多糖HEPF2,分子量大小为1.66×104Da,该多糖由岩藻糖、半乳糖和葡萄糖以1.00:3.69:5.42比例构成,同时也含有微量的3-O-甲基鼠李糖。进一步利用傅立叶变换红外光谱法、糖组成分析、甲基化分析、部分酸水解法和核磁共振法等方法进行结构鉴定,检测结果表明,该杂多糖中包含1→4、1→6结合的葡萄糖和1→6结合的半乳糖残基,连接于主链的侧链残基,包括岩藻糖残基、少数的端基葡萄糖和半乳糖残基。核磁共振法检测结果还表明,1→4结合葡萄糖为β构型,(1→6)结合半乳糖、(1→2,6)结合半乳糖和端基葡萄糖均为α构型。
A novel water-soluble heteropolysaccharide termed HEPF2 was isolated from the fruiting bodies of Hericium erinaceus.HEPF2 has a molecular weight of 1.66×104Da and is composed of fucose,galactose and glucose at a ratio of 1.00：3.69：5.42.HEPF2 also contains a small amount of 3-O-methylrhamnose.Its structural characteristics were further investigated by using combined technologies including FT-IR,sugar and methylation analysis,partial acid hydrolysis and NMR spectroscopy.The results showed that HEPF2 was a heteropolysaccharide chemically featured by（1→4）-linked glucosyl,（1→6）-linked glucosyl and（1→6）-linked galactosyl residue,attached to the O-2 of main chain with terminal fucosyl residue or a minor of terminal glucosyl and galactosyl residues.NMR spectra showed that（1→4）-linked glucosyl was β configuration,while（1→6）-linked galactosyl,（1→2,6）-linked galactosyl together with the terminal fucosyl residue were all α configuration.
出处
《菌物学报》
CAS
CSCD
北大核心
2010年第6期911-917,共7页
Mycosystema
基金
Supported by Leading Academic Discipline Project of Shanghai Municipal Education Commission(No.J50704)
关键词
大型真菌
传统中草药
杂多糖
纯化
结构分析
fungi
Chinese traditional herb
heteropolysaccharide
purification
structure elucidation
