摘要
本实验用琼脂凝胶免疫扩散试验（ＡＧＩＤＴ）和免疫印迹试验（ＩＢＡ）对实验感染绵羊进行性肺炎病毒（ＯＰＰＶ）的山羊血清与山羊关节炎—脑炎病毒（ＣＡＥＶ）抗原以及实验感染ＣＡＥＶ的绵羊血清与ＯＰＰＶ抗原的交叉反应进行了研究。４只接种ＯＰＰＶ的山羊中有一只山羊的血清可与ＣＡＥＶ琼扩抗原发生交叉反应，并在免疫印迹试验中可识别ＣＡＥＶ的ｇｐ４４、ｐ３５和ｐ２８。２只接种ＣＡＥＶ的绵羊中有一只绵羊的血清可与ＯＰＰＶ琼扩抗原发生交叉反应，并在免疫印迹试验中可识别ＯＰＰＶ的ｇｐ４４和ｐ２８。以上的交叉反应结果表明ＯＰＰＶ与ＣＡＥＶ的抗原之间具有密切的相关性，这对于ＯＰＰＶ通过山羊和ＣＡＥＶ通过绵羊的传代研究是非常重要的，并对将来的免疫预防策略具有重要的指导意义。
In this study cross reactivity between caprine arthritis encephalitis virus(CAEV)antigen and the sera of goats experimentally infected with ovine progressive pneumonia virus(OPPV)and OPPV antigen and the sera of sheep experimentally infected with CAEV was analyzed utilizing agar gel immunodiffusion test(AGIDT)and immunoblot assay(IBA) The sera of one of 4 goats inoculated with OPPV could react with agar gel immunodiffusion antigen of CAEV and recognize gp44,p35 and p28 of CAEV in IBA The sera of one of two sheep inoculated with CAEV could react with agar gel immunodiffusion antigen of OPPV and recognize gp44 and p28 of OPPV in IBA All above results indicate that there is a close relatedness between the antigens of OPPV and CAEV This is very important to the studies on passages of OPPV in goats and CAEV in sheep and has an important impact on the future vaccination strategies of ovine progressive pneumonia(OPP)and caprine arthritis encephalitis(CAE)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1999年第4期365-369,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
绵羊
进行性肺炎病毒
山羊
CAEV
交叉反应
Ovine progressive pneumonia virus,Caprine arthritis encephalitis virus,Cross reactivity
