摘要
目的:利用ITS序列对天门冬属9个物种进行分析,鉴定真假天门冬药材和分析天门冬属系统发育。方法:通过对ITS序列进行扩增、克隆测序及序列比对分析。结果:9物种的ITS序列全长为711～748 bp,G+C含量约为60%;以ITS全序列构建的系统树将9个种分为2个亚群:天门冬、石刁柏、武竹、非洲天门冬和狐尾天冬为1亚群,其他4个种为1个亚群;第1个亚群中,来自非洲的非洲天门冬和狐尾天冬优先聚类,然后与非洲天门冬的变种武竹聚为一类;另1亚群中,文竹与其他三种天门冬的亲缘关系相对较远。结论:ITS可以将不同天门冬属物种区分开来,但某些物种在系统树中的划分地位值得商榷,认为ITS序列最好结合其他多种分析手段才能更加准确的分析属内物种的系统发育。
Objective： Using ITS sequence of nine species to identify counterfeiting medicine and analyse phylogenetic of Asparagus.Methods： Analysing ITS sequences by amplification,cloning,sequencing and alignment.Results： The length range of ITS sequence of nine species was from 711 to 748 bp,the percentage of G ＋ C content was about 60%.The phylogenetic tree constructed on the basis of the ITS sequences showed that nine species were divided into two branches： Asparagus cochinchinensis,Asparagus officinalis,Asparagus densiflorus,Asparagus densiflorus cv.Myers and Asparagus densiflorus cv.Sprengeri were a branch and the others were a branch.Asparagus densiflorus and Asparagus densiflorus cv.Myers those were from Africa had priority to clustering and then clustering with Asparagus densiflorus cv.Sprengeri that was a variant of Asparagus densiflorus in the first branch.Asparagus setaceus had relatively distant genetic relationship with the others three materials in another branch.Conclusions： The ITS sequences could distinguish species of Asparagus to test the counterfeit.Division status in phylogenetic tree of some species were debatable and ITS sequence was combined with others analytical tools to analyze the realistic phylogeny.
出处
《中药材》
CAS
CSCD
北大核心
2010年第10期1542-1545,共4页
Journal of Chinese Medicinal Materials
基金
湖南省高校创新平台开放基金项目(09K106)
湖南省科技计划重点项目(2009FJ2008)
关键词
天门冬属
ITS序列
鉴定
系统发育
Asparagus L.
ITS sequence
Identification
Phylogenetic
